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Detecting stoichiometry of macromolecular complexes in live cells using FRET

The stoichiometry of macromolecular interactions is fundamental to cellular signalling yet challenging to detect from living cells. Fluorescence resonance energy transfer (FRET) is a powerful phenomenon for characterizing close-range interactions whereby a donor fluorophore transfers energy to a clo...

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Detalles Bibliográficos
Autores principales: Ben-Johny, Manu, Yue, Daniel N., Yue, David T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5150656/
https://www.ncbi.nlm.nih.gov/pubmed/27922011
http://dx.doi.org/10.1038/ncomms13709