Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing

The non-conventional yeast Pichia pastoris is a popular host for recombinant protein production in scientific research and industry. Typically, the expression cassette is integrated into the genome via homologous recombination. Due to unknown integration events, a large clonal variability is often e...

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Detalles Bibliográficos
Autores principales: Schwarzhans, Jan-Philipp, Wibberg, Daniel, Winkler, Anika, Luttermann, Tobias, Kalinowski, Jörn, Friehs, Karl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154183/
https://www.ncbi.nlm.nih.gov/pubmed/27958335
http://dx.doi.org/10.1038/srep38952

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154183/
https://www.ncbi.nlm.nih.gov/pubmed/27958335
http://dx.doi.org/10.1038/srep38952

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