Cargando…

RAB43 facilitates cross-presentation of cell-associated antigens by CD8α(+) dendritic cells

In this study, to examine cross-presentation by classical dendritic cells (DCs; cDCs), we evaluated the role of RAB43, a protein found to be selectively expressed by Batf3-dependent CD8α(+) and CD103(+) compared with other DC subsets and immune lineages. Using a specific monoclonal antibody, we loca...

Descripción completa

Detalles Bibliográficos
Autores principales: Kretzer, Nicole M., Theisen, Derek J., Tussiwand, Roxane, Briseño, Carlos G., Grajales-Reyes, Gary E., Wu, Xiaodi, Durai, Vivek, Albring, Jörn, Bagadia, Prachi, Murphy, Theresa L., Murphy, Kenneth M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154939/
https://www.ncbi.nlm.nih.gov/pubmed/27899443
http://dx.doi.org/10.1084/jem.20160597
_version_ 1782474917287034880
author Kretzer, Nicole M.
Theisen, Derek J.
Tussiwand, Roxane
Briseño, Carlos G.
Grajales-Reyes, Gary E.
Wu, Xiaodi
Durai, Vivek
Albring, Jörn
Bagadia, Prachi
Murphy, Theresa L.
Murphy, Kenneth M.
author_facet Kretzer, Nicole M.
Theisen, Derek J.
Tussiwand, Roxane
Briseño, Carlos G.
Grajales-Reyes, Gary E.
Wu, Xiaodi
Durai, Vivek
Albring, Jörn
Bagadia, Prachi
Murphy, Theresa L.
Murphy, Kenneth M.
author_sort Kretzer, Nicole M.
collection PubMed
description In this study, to examine cross-presentation by classical dendritic cells (DCs; cDCs), we evaluated the role of RAB43, a protein found to be selectively expressed by Batf3-dependent CD8α(+) and CD103(+) compared with other DC subsets and immune lineages. Using a specific monoclonal antibody, we localized RAB43 expression to the Golgi apparatus and LAMP1(−) cytoplasmic vesicles. Mice with germline or conditional deletion of Rab43 are viable and fertile and have normal development of cDCs but show a defect for in vivo and in vitro cross-presentation of cell-associated antigen. This defect is specific to cDCs, as Rab43-deficient monocyte-derived DCs showed no defect in cross-presentation of cell-associated antigen. These results suggest that RAB43 provides a specialized activity used in cross-presentation selectively by CD8α(+) DCs but not other antigen-presenting cells.
format Online
Article
Text
id pubmed-5154939
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-51549392017-06-12 RAB43 facilitates cross-presentation of cell-associated antigens by CD8α(+) dendritic cells Kretzer, Nicole M. Theisen, Derek J. Tussiwand, Roxane Briseño, Carlos G. Grajales-Reyes, Gary E. Wu, Xiaodi Durai, Vivek Albring, Jörn Bagadia, Prachi Murphy, Theresa L. Murphy, Kenneth M. J Exp Med Research Articles In this study, to examine cross-presentation by classical dendritic cells (DCs; cDCs), we evaluated the role of RAB43, a protein found to be selectively expressed by Batf3-dependent CD8α(+) and CD103(+) compared with other DC subsets and immune lineages. Using a specific monoclonal antibody, we localized RAB43 expression to the Golgi apparatus and LAMP1(−) cytoplasmic vesicles. Mice with germline or conditional deletion of Rab43 are viable and fertile and have normal development of cDCs but show a defect for in vivo and in vitro cross-presentation of cell-associated antigen. This defect is specific to cDCs, as Rab43-deficient monocyte-derived DCs showed no defect in cross-presentation of cell-associated antigen. These results suggest that RAB43 provides a specialized activity used in cross-presentation selectively by CD8α(+) DCs but not other antigen-presenting cells. The Rockefeller University Press 2016-12-12 /pmc/articles/PMC5154939/ /pubmed/27899443 http://dx.doi.org/10.1084/jem.20160597 Text en © 2016 Kretzer et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Kretzer, Nicole M.
Theisen, Derek J.
Tussiwand, Roxane
Briseño, Carlos G.
Grajales-Reyes, Gary E.
Wu, Xiaodi
Durai, Vivek
Albring, Jörn
Bagadia, Prachi
Murphy, Theresa L.
Murphy, Kenneth M.
RAB43 facilitates cross-presentation of cell-associated antigens by CD8α(+) dendritic cells
title RAB43 facilitates cross-presentation of cell-associated antigens by CD8α(+) dendritic cells
title_full RAB43 facilitates cross-presentation of cell-associated antigens by CD8α(+) dendritic cells
title_fullStr RAB43 facilitates cross-presentation of cell-associated antigens by CD8α(+) dendritic cells
title_full_unstemmed RAB43 facilitates cross-presentation of cell-associated antigens by CD8α(+) dendritic cells
title_short RAB43 facilitates cross-presentation of cell-associated antigens by CD8α(+) dendritic cells
title_sort rab43 facilitates cross-presentation of cell-associated antigens by cd8α(+) dendritic cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154939/
https://www.ncbi.nlm.nih.gov/pubmed/27899443
http://dx.doi.org/10.1084/jem.20160597
work_keys_str_mv AT kretzernicolem rab43facilitatescrosspresentationofcellassociatedantigensbycd8adendriticcells
AT theisenderekj rab43facilitatescrosspresentationofcellassociatedantigensbycd8adendriticcells
AT tussiwandroxane rab43facilitatescrosspresentationofcellassociatedantigensbycd8adendriticcells
AT brisenocarlosg rab43facilitatescrosspresentationofcellassociatedantigensbycd8adendriticcells
AT grajalesreyesgarye rab43facilitatescrosspresentationofcellassociatedantigensbycd8adendriticcells
AT wuxiaodi rab43facilitatescrosspresentationofcellassociatedantigensbycd8adendriticcells
AT duraivivek rab43facilitatescrosspresentationofcellassociatedantigensbycd8adendriticcells
AT albringjorn rab43facilitatescrosspresentationofcellassociatedantigensbycd8adendriticcells
AT bagadiaprachi rab43facilitatescrosspresentationofcellassociatedantigensbycd8adendriticcells
AT murphytheresal rab43facilitatescrosspresentationofcellassociatedantigensbycd8adendriticcells
AT murphykennethm rab43facilitatescrosspresentationofcellassociatedantigensbycd8adendriticcells