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Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods

BACKGROUND: High-resolution 3D imaging of intact tissue facilitates cellular and subcellular analyses of complex structures within their native environment. However, difficulties associated with immunolabelling and imaging fluorescent proteins deep within whole organs have restricted their applicati...

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Detalles Bibliográficos
Autores principales: Lloyd-Lewis, Bethan, Davis, Felicity M., Harris, Olivia B., Hitchcock, Jessica R., Lourenco, Filipe C., Pasche, Mathias, Watson, Christine J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155399/
https://www.ncbi.nlm.nih.gov/pubmed/27964754
http://dx.doi.org/10.1186/s13058-016-0754-9