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Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods
BACKGROUND: High-resolution 3D imaging of intact tissue facilitates cellular and subcellular analyses of complex structures within their native environment. However, difficulties associated with immunolabelling and imaging fluorescent proteins deep within whole organs have restricted their applicati...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155399/ https://www.ncbi.nlm.nih.gov/pubmed/27964754 http://dx.doi.org/10.1186/s13058-016-0754-9 |