Use of Fluorescence Lifetime Imaging Microscopy (FLIM) as a Timer of Cell Cycle S Phase

Incorporation of thymidine analogues in replicating DNA, coupled with antibody and fluorophore staining, allows analysis of cell proliferation, but is currently limited to monolayer cultures, fixed cells and end-point assays. We describe a simple microscopy imaging method for live real-time analysis...

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Detalles Bibliográficos
Autores principales: Okkelman, Irina A., Dmitriev, Ruslan I., Foley, Tara, Papkovsky, Dmitri B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5156356/
https://www.ncbi.nlm.nih.gov/pubmed/27973570
http://dx.doi.org/10.1371/journal.pone.0167385

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5156356/
https://www.ncbi.nlm.nih.gov/pubmed/27973570
http://dx.doi.org/10.1371/journal.pone.0167385

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