Calcineurin B-like Protein CBL10 Directly Interacts with TOC34 (Translocon of the Outer Membrane of the Chloroplasts) and Decreases Its GTPase Activity in Arabidopsis
As calcium sensor relays in plants, calcineurin B-like (CBL) proteins provide an important contribution to decoding Ca(2+) signatures elicited by a variety of abiotic stresses. Currently, it is well known that CBLs perceive and transmit the Ca(2+) signals mainly to a group of serine/threonine protei...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5156837/ https://www.ncbi.nlm.nih.gov/pubmed/28018422 http://dx.doi.org/10.3389/fpls.2016.01911 |
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author | Cho, Joo Hyuk Lee, Jeong Hwan Park, Yoon Kook Choi, Mi Na Kim, Kyung-Nam |
author_facet | Cho, Joo Hyuk Lee, Jeong Hwan Park, Yoon Kook Choi, Mi Na Kim, Kyung-Nam |
author_sort | Cho, Joo Hyuk |
collection | PubMed |
description | As calcium sensor relays in plants, calcineurin B-like (CBL) proteins provide an important contribution to decoding Ca(2+) signatures elicited by a variety of abiotic stresses. Currently, it is well known that CBLs perceive and transmit the Ca(2+) signals mainly to a group of serine/threonine protein kinases called CBL-interacting protein kinases (CIPKs). In this study, we report that the CBL10 member of this family has a novel interaction partner besides the CIPK proteins. Yeast two-hybrid screening with CBL10 as bait identified an Arabidopsis cDNA clone encoding a TOC34 protein, which is a member of the TOC (Translocon of the Outer membrane of the Chloroplasts) complex and possesses the GTPase activity. Further analyses showed that in addition to CBL10, CBL7 also interacts with TOC34 at much lower strength in the yeast two-hybrid system. However, the rest of the CBL family members failed to interact with TOC34. Bimolecular fluorescence complementation (BiFC) analysis verified that the CBL10-TOC34 interaction occurs at the outer membrane of chloroplasts in vivo. In addition, we also demonstrated that CBL10 physically associates with TOC34 in vitro, resulting in a significant decrease in the GTPase activity of the TOC34 protein. Taken together, our findings clearly indicate that a member of the CBL family, CBL10, can modulate not only the CIPK members but also TOC34, allowing the CBL family to relay the Ca(2+) signals in more diverse ways than currently known. |
format | Online Article Text |
id | pubmed-5156837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-51568372016-12-23 Calcineurin B-like Protein CBL10 Directly Interacts with TOC34 (Translocon of the Outer Membrane of the Chloroplasts) and Decreases Its GTPase Activity in Arabidopsis Cho, Joo Hyuk Lee, Jeong Hwan Park, Yoon Kook Choi, Mi Na Kim, Kyung-Nam Front Plant Sci Plant Science As calcium sensor relays in plants, calcineurin B-like (CBL) proteins provide an important contribution to decoding Ca(2+) signatures elicited by a variety of abiotic stresses. Currently, it is well known that CBLs perceive and transmit the Ca(2+) signals mainly to a group of serine/threonine protein kinases called CBL-interacting protein kinases (CIPKs). In this study, we report that the CBL10 member of this family has a novel interaction partner besides the CIPK proteins. Yeast two-hybrid screening with CBL10 as bait identified an Arabidopsis cDNA clone encoding a TOC34 protein, which is a member of the TOC (Translocon of the Outer membrane of the Chloroplasts) complex and possesses the GTPase activity. Further analyses showed that in addition to CBL10, CBL7 also interacts with TOC34 at much lower strength in the yeast two-hybrid system. However, the rest of the CBL family members failed to interact with TOC34. Bimolecular fluorescence complementation (BiFC) analysis verified that the CBL10-TOC34 interaction occurs at the outer membrane of chloroplasts in vivo. In addition, we also demonstrated that CBL10 physically associates with TOC34 in vitro, resulting in a significant decrease in the GTPase activity of the TOC34 protein. Taken together, our findings clearly indicate that a member of the CBL family, CBL10, can modulate not only the CIPK members but also TOC34, allowing the CBL family to relay the Ca(2+) signals in more diverse ways than currently known. Frontiers Media S.A. 2016-12-15 /pmc/articles/PMC5156837/ /pubmed/28018422 http://dx.doi.org/10.3389/fpls.2016.01911 Text en Copyright © 2016 Cho, Lee, Park, Choi and Kim. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Cho, Joo Hyuk Lee, Jeong Hwan Park, Yoon Kook Choi, Mi Na Kim, Kyung-Nam Calcineurin B-like Protein CBL10 Directly Interacts with TOC34 (Translocon of the Outer Membrane of the Chloroplasts) and Decreases Its GTPase Activity in Arabidopsis |
title | Calcineurin B-like Protein CBL10 Directly Interacts with TOC34 (Translocon of the Outer Membrane of the Chloroplasts) and Decreases Its GTPase Activity in Arabidopsis |
title_full | Calcineurin B-like Protein CBL10 Directly Interacts with TOC34 (Translocon of the Outer Membrane of the Chloroplasts) and Decreases Its GTPase Activity in Arabidopsis |
title_fullStr | Calcineurin B-like Protein CBL10 Directly Interacts with TOC34 (Translocon of the Outer Membrane of the Chloroplasts) and Decreases Its GTPase Activity in Arabidopsis |
title_full_unstemmed | Calcineurin B-like Protein CBL10 Directly Interacts with TOC34 (Translocon of the Outer Membrane of the Chloroplasts) and Decreases Its GTPase Activity in Arabidopsis |
title_short | Calcineurin B-like Protein CBL10 Directly Interacts with TOC34 (Translocon of the Outer Membrane of the Chloroplasts) and Decreases Its GTPase Activity in Arabidopsis |
title_sort | calcineurin b-like protein cbl10 directly interacts with toc34 (translocon of the outer membrane of the chloroplasts) and decreases its gtpase activity in arabidopsis |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5156837/ https://www.ncbi.nlm.nih.gov/pubmed/28018422 http://dx.doi.org/10.3389/fpls.2016.01911 |
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