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Propidium Monoazide Integrated with qPCR Enables the Detection and Enumeration of Infectious Enteric RNA and DNA Viruses in Clam and Fermented Sausages

The increase of foodborne viral outbreaks highlights the need for a rapid and sensitive method for the prediction of viral infectivity in food samples. This study assesses the use of propidium monoazide (PMA) coupled with real-time PCR methods (RT-qPCR or qPCR for RNA or DNA viruses, respectively) i...

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Detalles Bibliográficos
Autores principales: Quijada, Narciso M., Fongaro, Gislaine, Barardi, Célia R. M., Hernández, Marta, Rodríguez-Lázaro, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5156952/
https://www.ncbi.nlm.nih.gov/pubmed/28018329
http://dx.doi.org/10.3389/fmicb.2016.02008