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Propidium Monoazide Integrated with qPCR Enables the Detection and Enumeration of Infectious Enteric RNA and DNA Viruses in Clam and Fermented Sausages
The increase of foodborne viral outbreaks highlights the need for a rapid and sensitive method for the prediction of viral infectivity in food samples. This study assesses the use of propidium monoazide (PMA) coupled with real-time PCR methods (RT-qPCR or qPCR for RNA or DNA viruses, respectively) i...
Autores principales: | Quijada, Narciso M., Fongaro, Gislaine, Barardi, Célia R. M., Hernández, Marta, Rodríguez-Lázaro, David |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5156952/ https://www.ncbi.nlm.nih.gov/pubmed/28018329 http://dx.doi.org/10.3389/fmicb.2016.02008 |
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