Cargando…

Single-molecule analysis of ϕC31 integrase-mediated site-specific recombination by tethered particle motion

Serine and tyrosine site-specific recombinases (SRs and YRs, respectively) provide templates for understanding the chemical mechanisms and conformational dynamics of strand cleavage/exchange between DNA partners. Current evidence suggests a rather intriguing mechanism for serine recombination, in wh...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fan, Hsiu-Fang, Hsieh, Tao-shih, Ma, Chien-Hui, Jayaram, Makkuni
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2016
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159548/ https://www.ncbi.nlm.nih.gov/pubmed/27986956 http://dx.doi.org/10.1093/nar/gkw861

Ejemplares similares

Real-time single-molecule tethered particle motion analysis reveals mechanistic similarities and contrasts of Flp site-specific recombinase with Cre and λ Int
por: Fan, Hsiu-Fang, et al.
Publicado: (2013)

A motif in the C-terminal domain of ϕC31 integrase controls the directionality of recombination
por: Rowley, Paul A., et al.
Publicado: (2008)

Cell type differences in activity of the Streptomyces bacteriophage ϕC31 integrase
por: Maucksch, Christof, et al.
Publicado: (2008)

The mechanism of ϕC31 integrase directionality: experimental analysis and computational modelling
por: Pokhilko, Alexandra, et al.
Publicado: (2016)

DNA binding and synapsis by the large C-terminal domain of ϕC31 integrase
por: McEwan, Andrew R., et al.
Publicado: (2009)

Real-time single-molecule tethered particle motion experiments reveal the kinetics and mechanisms of Cre-mediated site-specific recombination
por: Fan, Hsiu-Fang
Publicado: (2012)

Zinc is essential for high-affinity DNA binding and recombinase activity of φC31 integrase
por: McEwan, Andrew R., et al.
Publicado: (2011)

Single molecule TPM analysis of the catalytic pentad mutants of Cre and Flp site-specific recombinases: contributions of the pentad residues to the pre-chemical steps of recombination
por: Fan, Hsiu-Fang, et al.
Publicado: (2015)

The Xer activation factor of TLCΦ expands the possibilities for Xer recombination
por: Miele, Solange, et al.
Publicado: (2022)

Single-Molecule Tethered Particle Motion: Stepwise Analyses of Site-Specific DNA Recombination
por: Fan, Hsiu-Fang, et al.
Publicado: (2018)

Restoration of catalytic functions in Cre recombinase mutants by electrostatic compensation between active site and DNA substrate
por: Kachroo, Aashiq H., et al.
Publicado: (2010)

Identification of key structural determinants of the IntI1 integron integrase that influence attC × attI1 recombination efficiency
por: Demarre, Gaëlle, et al.
Publicado: (2007)

Involvement of the TPR2 subdomain movement in the activities of ϕ29 DNA polymerase
por: Rodríguez, Irene, et al.
Publicado: (2009)

Sequence motifs recognized by the casposon integrase of Aciduliprofundum boonei
por: Béguin, Pierre, et al.
Publicado: (2019)

Coiled-coil interactions mediate serine integrase directionality
por: Gupta, Kushol, et al.
Publicado: (2017)

Dynamics on multiple timescales in the RNA-directed RNA polymerase from the cystovirus ϕ6
por: Ren, Zhen, et al.
Publicado: (2010)

Spermidine biases the resolution of Holliday junctions by phage λ integrase
por: Boldt, Jeffrey L., et al.
Publicado: (2007)

Functional and structural characterization of the integrase from the prototype foamy virus
por: Valkov, Eugene, et al.
Publicado: (2009)

A bipartite thermodynamic-kinetic contribution by an activating mutation to RDF-independent excision by a phage serine integrase
por: Fan, Hsiu-Fang, et al.
Publicado: (2020)

LEDGF/p75 interacts with divergent lentiviral integrases and modulates their enzymatic activity in vitro
por: Cherepanov, Peter
Publicado: (2007)

CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges
por: Malanowska, Karolina, et al.
Publicado: (2007)

Modulation of the intrinsic chromatin binding property of HIV-1 integrase by LEDGF/p75
por: Lapaillerie, Delphine, et al.
Publicado: (2021)

B′-protein phosphatase 2A is a functional binding partner of delta-retroviral integrase
por: Maertens, Goedele N.
Publicado: (2016)

Involvement of phage ϕ29 DNA polymerase and terminal protein subdomains in conferring specificity during initiation of protein-primed DNA replication
por: Pérez-Arnaiz, Patricia, et al.
Publicado: (2007)

Insights into the pre-initiation events of bacteriophage φ6 RNA-dependent RNA polymerase: towards the assembly of a productive binary complex
por: Sarin, L. Peter, et al.
Publicado: (2009)

Role of metal ions in catalysis by HIV integrase analyzed using a quantitative PCR disintegration assay
por: Diamond, Tracy L., et al.
Publicado: (2006)

Resolution of the Holliday junction recombination intermediate by human GEN1 at the single-molecule level
por: Sobhy, Mohamed A, et al.
Publicado: (2019)

In vitro initial attachment of HIV-1 integrase to viral ends: control of the DNA specific interaction by the oligomerization state
por: Lesbats, P., et al.
Publicado: (2008)

Integrase residues that determine nucleotide preferences at sites of HIV-1 integration: implications for the mechanism of target DNA binding
por: Serrao, Erik, et al.
Publicado: (2014)

Human H4 tail stimulates HIV-1 integration through binding to the carboxy-terminal domain of integrase
por: Mauro, Eric, et al.
Publicado: (2019)

The casposon-encoded Cas1 protein from Aciduliprofundum boonei is a DNA integrase that generates target site duplications
por: Hickman, Alison B., et al.
Publicado: (2015)

DNA binding specificities of Escherichia coli Cas1–Cas2 integrase drive its recruitment at the CRISPR locus
por: Moch, Clara, et al.
Publicado: (2017)

The G140S mutation in HIV integrases from raltegravir-resistant patients rescues catalytic defect due to the resistance Q148H mutation
por: Delelis, Olivier, et al.
Publicado: (2009)

A cooperative and specific DNA-binding mode of HIV-1 integrase depends on the nature of the metallic cofactor and involves the zinc-containing N-terminal domain
por: Carayon, Kevin, et al.
Publicado: (2010)

Selectivity for strand-transfer over 3′-processing and susceptibility to clinical resistance of HIV-1 integrase inhibitors are driven by key enzyme–DNA interactions in the active site
por: Métifiot, Mathieu, et al.
Publicado: (2016)

Nicked-site substrates for a serine recombinase reveal enzyme–DNA communications and an essential tethering role of covalent enzyme–DNA linkages
por: Olorunniji, Femi J., et al.
Publicado: (2015)

The monomeric GIY-YIG homing endonuclease I-BmoI uses a molecular anchor and a flexible tether to sequentially nick DNA
por: Kleinstiver, Benjamin P., et al.
Publicado: (2013)

Asymmetric DNA requirements in Xer recombination activation by FtsK
por: Bonné, Laetitia, et al.
Publicado: (2009)

Measurements of DNA-loop formation via Cre-mediated recombination
por: Shoura, Massa J., et al.
Publicado: (2012)

Biochemical and genetic analysis of the role of the viral polymerase in enterovirus recombination
por: Woodman, Andrew, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_3ogslqs5b16ket07la7ja8up14