Methylation-Sensitive Melt Curve Analysis of the Reprimo Gene Methylation in Gastric Cancer

Reprimo (RPRM) is a p53-induced tumor suppressor gene. Its aberrant DNA methylation is correlated with carcinogenesis and may be used as a surrogate marker for the early detection of gastric cancer. However, the detail information regarding its DNA methylation has not been revealed. Here, we investi...

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Autores principales: Wang, Hanze, Zheng, Yansong, Lai, Junzhong, Luo, Qianping, Ke, Huican, Chen, Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5161478/
https://www.ncbi.nlm.nih.gov/pubmed/27992600
http://dx.doi.org/10.1371/journal.pone.0168635
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author Wang, Hanze
Zheng, Yansong
Lai, Junzhong
Luo, Qianping
Ke, Huican
Chen, Qi
author_facet Wang, Hanze
Zheng, Yansong
Lai, Junzhong
Luo, Qianping
Ke, Huican
Chen, Qi
author_sort Wang, Hanze
collection PubMed
description Reprimo (RPRM) is a p53-induced tumor suppressor gene. Its aberrant DNA methylation is correlated with carcinogenesis and may be used as a surrogate marker for the early detection of gastric cancer. However, the detail information regarding its DNA methylation has not been revealed. Here, we investigated the RPRM gene methylation in gastric cancer tumor and plasma samples by methylation-sensitive melt curve analysis (MS-MCA) and bisulfite sequencing in depth. We developed a semi-quantitative method based on MS-MCA for detecting DNA methylation and unraveled the RPRM gene methylation pattern in gastric cancer. This study provides a solid foundation for the future application of detecting RPRM gene methylation in human plasma or serum samples to help diagnose gastric cancer or for prognosis evaluation.
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spelling pubmed-51614782017-01-04 Methylation-Sensitive Melt Curve Analysis of the Reprimo Gene Methylation in Gastric Cancer Wang, Hanze Zheng, Yansong Lai, Junzhong Luo, Qianping Ke, Huican Chen, Qi PLoS One Research Article Reprimo (RPRM) is a p53-induced tumor suppressor gene. Its aberrant DNA methylation is correlated with carcinogenesis and may be used as a surrogate marker for the early detection of gastric cancer. However, the detail information regarding its DNA methylation has not been revealed. Here, we investigated the RPRM gene methylation in gastric cancer tumor and plasma samples by methylation-sensitive melt curve analysis (MS-MCA) and bisulfite sequencing in depth. We developed a semi-quantitative method based on MS-MCA for detecting DNA methylation and unraveled the RPRM gene methylation pattern in gastric cancer. This study provides a solid foundation for the future application of detecting RPRM gene methylation in human plasma or serum samples to help diagnose gastric cancer or for prognosis evaluation. Public Library of Science 2016-12-16 /pmc/articles/PMC5161478/ /pubmed/27992600 http://dx.doi.org/10.1371/journal.pone.0168635 Text en © 2016 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wang, Hanze
Zheng, Yansong
Lai, Junzhong
Luo, Qianping
Ke, Huican
Chen, Qi
Methylation-Sensitive Melt Curve Analysis of the Reprimo Gene Methylation in Gastric Cancer
title Methylation-Sensitive Melt Curve Analysis of the Reprimo Gene Methylation in Gastric Cancer
title_full Methylation-Sensitive Melt Curve Analysis of the Reprimo Gene Methylation in Gastric Cancer
title_fullStr Methylation-Sensitive Melt Curve Analysis of the Reprimo Gene Methylation in Gastric Cancer
title_full_unstemmed Methylation-Sensitive Melt Curve Analysis of the Reprimo Gene Methylation in Gastric Cancer
title_short Methylation-Sensitive Melt Curve Analysis of the Reprimo Gene Methylation in Gastric Cancer
title_sort methylation-sensitive melt curve analysis of the reprimo gene methylation in gastric cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5161478/
https://www.ncbi.nlm.nih.gov/pubmed/27992600
http://dx.doi.org/10.1371/journal.pone.0168635
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