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Can terminators be used as insulators into yeast synthetic gene circuits?

BACKGROUND: In bacteria, transcription units can be insulated by placing a terminator in front of a promoter. In this way promoter leakage due to the read-through from an upstream gene or RNA polymerase unspecific binding to the DNA is, in principle, removed. Differently from bacterial terminators,...

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Detalles Bibliográficos
Autores principales: Song, Wenjiang, Li, Jing, Liang, Qiang, Marchisio, Mario Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5162094/
https://www.ncbi.nlm.nih.gov/pubmed/28018483
http://dx.doi.org/10.1186/s13036-016-0040-5