Identification of G-quadruplexes in long functional RNAs using 7-deaza-Guanine RNA

RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences within long functional RNA molecules. We have developed a novel strategy, footprin...

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Autores principales: Weldon, Carika, Behm-Ansmant, Isabelle, Hurley, Laurence H., Burley, Glenn A., Branlant, Christiane, Eperon, Ian C., Dominguez, Cyril
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5164935/
https://www.ncbi.nlm.nih.gov/pubmed/27820800
http://dx.doi.org/10.1038/nchembio.2228
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author Weldon, Carika
Behm-Ansmant, Isabelle
Hurley, Laurence H.
Burley, Glenn A.
Branlant, Christiane
Eperon, Ian C.
Dominguez, Cyril
author_facet Weldon, Carika
Behm-Ansmant, Isabelle
Hurley, Laurence H.
Burley, Glenn A.
Branlant, Christiane
Eperon, Ian C.
Dominguez, Cyril
author_sort Weldon, Carika
collection PubMed
description RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences within long functional RNA molecules. We have developed a novel strategy, footprinting of long 7-deazaguanine substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and in functional conditions.
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spelling pubmed-51649352017-05-07 Identification of G-quadruplexes in long functional RNAs using 7-deaza-Guanine RNA Weldon, Carika Behm-Ansmant, Isabelle Hurley, Laurence H. Burley, Glenn A. Branlant, Christiane Eperon, Ian C. Dominguez, Cyril Nat Chem Biol Article RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences within long functional RNA molecules. We have developed a novel strategy, footprinting of long 7-deazaguanine substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and in functional conditions. 2016-11-07 2017-01 /pmc/articles/PMC5164935/ /pubmed/27820800 http://dx.doi.org/10.1038/nchembio.2228 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Weldon, Carika
Behm-Ansmant, Isabelle
Hurley, Laurence H.
Burley, Glenn A.
Branlant, Christiane
Eperon, Ian C.
Dominguez, Cyril
Identification of G-quadruplexes in long functional RNAs using 7-deaza-Guanine RNA
title Identification of G-quadruplexes in long functional RNAs using 7-deaza-Guanine RNA
title_full Identification of G-quadruplexes in long functional RNAs using 7-deaza-Guanine RNA
title_fullStr Identification of G-quadruplexes in long functional RNAs using 7-deaza-Guanine RNA
title_full_unstemmed Identification of G-quadruplexes in long functional RNAs using 7-deaza-Guanine RNA
title_short Identification of G-quadruplexes in long functional RNAs using 7-deaza-Guanine RNA
title_sort identification of g-quadruplexes in long functional rnas using 7-deaza-guanine rna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5164935/
https://www.ncbi.nlm.nih.gov/pubmed/27820800
http://dx.doi.org/10.1038/nchembio.2228
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