A Systematic Investigation of Parameters Influencing Droplet Rain in the Listeria monocytogenes prfA Assay - Reduction of Ambiguous Results in ddPCR

The droplet digital polymerase chain reaction (ddPCR) determines DNA amounts based upon the pattern of positive and negative droplets, according to Poisson distribution, without the use of external standards. However, division into positive and negative droplets is often not clear because a part of...

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Detalles Bibliográficos
Autores principales: Witte, Anna Kristina, Mester, Patrick, Fister, Susanne, Witte, Matthias, Schoder, Dagmar, Rossmanith, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5167268/
https://www.ncbi.nlm.nih.gov/pubmed/27992475
http://dx.doi.org/10.1371/journal.pone.0168179

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5167268/
https://www.ncbi.nlm.nih.gov/pubmed/27992475
http://dx.doi.org/10.1371/journal.pone.0168179

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