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A Systematic Investigation of Parameters Influencing Droplet Rain in the Listeria monocytogenes prfA Assay - Reduction of Ambiguous Results in ddPCR
The droplet digital polymerase chain reaction (ddPCR) determines DNA amounts based upon the pattern of positive and negative droplets, according to Poisson distribution, without the use of external standards. However, division into positive and negative droplets is often not clear because a part of...
Autores principales: | Witte, Anna Kristina, Mester, Patrick, Fister, Susanne, Witte, Matthias, Schoder, Dagmar, Rossmanith, Peter |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5167268/ https://www.ncbi.nlm.nih.gov/pubmed/27992475 http://dx.doi.org/10.1371/journal.pone.0168179 |
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