Modeling Enzyme Processivity Reveals that RNA-Seq Libraries Are Biased in Characteristic and Correctable Ways

Experimental procedures for preparing RNA-seq and single-cell (sc) RNA-seq libraries are based on assumptions regarding their underlying enzymatic reactions. Here, we show that the fairness of these assumptions varies within libraries: coverage by sequencing reads along and between transcripts exhib...

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Detalles Bibliográficos
Autores principales: Archer, Nathan, Walsh, Mark D., Shahrezaei, Vahid, Hebenstreit, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5167349/
https://www.ncbi.nlm.nih.gov/pubmed/27840077
http://dx.doi.org/10.1016/j.cels.2016.10.012

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5167349/
https://www.ncbi.nlm.nih.gov/pubmed/27840077
http://dx.doi.org/10.1016/j.cels.2016.10.012

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