Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR

BACKGROUND: Plasmids are widely used for molecular cloning or production of proteins in laboratory and industrial settings. Constant modification has brought forth countless plasmid vectors whose characteristics in terms of average plasmid copy number (PCN) and stability are rarely known. The crucia...

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Detalles Bibliográficos
Autores principales: Jahn, Michael, Vorpahl, Carsten, Hübschmann, Thomas, Harms, Hauke, Müller, Susann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5168713/
https://www.ncbi.nlm.nih.gov/pubmed/27993152
http://dx.doi.org/10.1186/s12934-016-0610-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5168713/
https://www.ncbi.nlm.nih.gov/pubmed/27993152
http://dx.doi.org/10.1186/s12934-016-0610-8

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