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Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR
BACKGROUND: Plasmids are widely used for molecular cloning or production of proteins in laboratory and industrial settings. Constant modification has brought forth countless plasmid vectors whose characteristics in terms of average plasmid copy number (PCN) and stability are rarely known. The crucia...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5168713/ https://www.ncbi.nlm.nih.gov/pubmed/27993152 http://dx.doi.org/10.1186/s12934-016-0610-8 |