Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9

CRISPR-Cas9 is efficient enough to knock out both alleles directly by introducing out-of-frame mutations. We succeeded in making biallelic on-target frameshift mutations of the endogenous Gli3 gene; however, the GLI3 protein was expressed in all six of the established cell lines carrying homozygous...

Descripción completa

Detalles Bibliográficos
Autores principales: Makino, Shigeru, Fukumura, Ryutaro, Gondo, Yoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5175197/
https://www.ncbi.nlm.nih.gov/pubmed/28000783
http://dx.doi.org/10.1038/srep39608

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5175197/
https://www.ncbi.nlm.nih.gov/pubmed/28000783
http://dx.doi.org/10.1038/srep39608

Ejemplares similares