A Rapid Spin Column-Based Method to Enrich Pathogen Transcripts from Eukaryotic Host Cells Prior to Sequencing

When analyzing pathogen transcriptomes during the infection of host cells, the signal-to-background (pathogen-to-host) ratio of nucleic acids (NA) in infected samples is very small. Despite the advancements in next-generation sequencing, the minute amount of pathogen NA makes standard RNA-seq librar...

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Detalles Bibliográficos
Autores principales: Bent, Zachary W., Poorey, Kunal, LaBauve, Annette E., Hamblin, Rachelle, Williams, Kelly P., Meagher, Robert J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5176299/
https://www.ncbi.nlm.nih.gov/pubmed/28002481
http://dx.doi.org/10.1371/journal.pone.0168788

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5176299/
https://www.ncbi.nlm.nih.gov/pubmed/28002481
http://dx.doi.org/10.1371/journal.pone.0168788

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