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Dual observation of the ATP-evoked small GTPase activation and Ca(2+) transient in astrocytes using a dark red fluorescent protein

Intracellular signal transduction involves a number of biochemical reactions, which largely consist of protein-protein interactions and protein conformational changes. Monitoring Förster resonance energy transfer (FRET) by fluorescence lifetime imaging microscopy (FLIM), called FLIM-FRET, is one of...

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Detalles Bibliográficos
Autores principales: Nakahata, Yoshihisa, Nabekura, Junichi, Murakoshi, Hideji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5177924/
https://www.ncbi.nlm.nih.gov/pubmed/28004840
http://dx.doi.org/10.1038/srep39564