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Dual observation of the ATP-evoked small GTPase activation and Ca(2+) transient in astrocytes using a dark red fluorescent protein
Intracellular signal transduction involves a number of biochemical reactions, which largely consist of protein-protein interactions and protein conformational changes. Monitoring Förster resonance energy transfer (FRET) by fluorescence lifetime imaging microscopy (FLIM), called FLIM-FRET, is one of...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5177924/ https://www.ncbi.nlm.nih.gov/pubmed/28004840 http://dx.doi.org/10.1038/srep39564 |