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Fluid flow facilitates inward rectifier K(+) current by convectively restoring [K(+)] at the cell membrane surface
The inward rectifier Kir2.1 current (IKir2.1) was reported to be facilitated by fluid flow. However, the mechanism underlying this facilitation remains uncertain. We hypothesized that during K(+) influx or efflux, [K(+)] adjacent to the outer mouth of the Kir2.1 channel might decrease or increase, r...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5177964/ https://www.ncbi.nlm.nih.gov/pubmed/28004830 http://dx.doi.org/10.1038/srep39585 |
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author | Kim, Jae Gon Park, Sang Woong Byun, Doyoung Choi, Wahn Soo Sung, Dong Jun Shin, Kyung Chul Kim, Hyun-ji Leem, Young-Eun Kang, Jong-Sun Cho, Hana Kim, Bokyung Cho, Sung I Bae, Young Min |
author_facet | Kim, Jae Gon Park, Sang Woong Byun, Doyoung Choi, Wahn Soo Sung, Dong Jun Shin, Kyung Chul Kim, Hyun-ji Leem, Young-Eun Kang, Jong-Sun Cho, Hana Kim, Bokyung Cho, Sung I Bae, Young Min |
author_sort | Kim, Jae Gon |
collection | PubMed |
description | The inward rectifier Kir2.1 current (IKir2.1) was reported to be facilitated by fluid flow. However, the mechanism underlying this facilitation remains uncertain. We hypothesized that during K(+) influx or efflux, [K(+)] adjacent to the outer mouth of the Kir2.1 channel might decrease or increase, respectively, compared with the average [K(+)] of the bulk extracellular solution, and that fluid flow could restore the original [K(+)] and result in the apparent facilitation of IKir2.1. We recorded the IKir2.1 in RBL-2H3 cells and HEK293T cells that were ectopically over-expressed with Kir2.1 channels by using the whole-cell patch-clamp technique. Fluid-flow application immediately increased the IKir2.1, which was not prevented by either the pretreatment with inhibitors of various protein kinases or the modulation of the cytoskeleton and caveolae. The magnitudes of the increases of IKir2.1 by fluid flow were driving force-dependent. Simulations performed using the Nernst-Planck mass equation indicated that [K(+)] near the membrane surface fell markedly below the average [K(+)] of the bulk extracellular solution during K(+) influx, and, notably, that fluid flow restored the decreased [K(+)] at the cell surface in a flow rate-dependent manner. These results support the “convection-regulation hypothesis” and define a novel interpretation of fluid flow-induced modulation of ion channels. |
format | Online Article Text |
id | pubmed-5177964 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51779642016-12-29 Fluid flow facilitates inward rectifier K(+) current by convectively restoring [K(+)] at the cell membrane surface Kim, Jae Gon Park, Sang Woong Byun, Doyoung Choi, Wahn Soo Sung, Dong Jun Shin, Kyung Chul Kim, Hyun-ji Leem, Young-Eun Kang, Jong-Sun Cho, Hana Kim, Bokyung Cho, Sung I Bae, Young Min Sci Rep Article The inward rectifier Kir2.1 current (IKir2.1) was reported to be facilitated by fluid flow. However, the mechanism underlying this facilitation remains uncertain. We hypothesized that during K(+) influx or efflux, [K(+)] adjacent to the outer mouth of the Kir2.1 channel might decrease or increase, respectively, compared with the average [K(+)] of the bulk extracellular solution, and that fluid flow could restore the original [K(+)] and result in the apparent facilitation of IKir2.1. We recorded the IKir2.1 in RBL-2H3 cells and HEK293T cells that were ectopically over-expressed with Kir2.1 channels by using the whole-cell patch-clamp technique. Fluid-flow application immediately increased the IKir2.1, which was not prevented by either the pretreatment with inhibitors of various protein kinases or the modulation of the cytoskeleton and caveolae. The magnitudes of the increases of IKir2.1 by fluid flow were driving force-dependent. Simulations performed using the Nernst-Planck mass equation indicated that [K(+)] near the membrane surface fell markedly below the average [K(+)] of the bulk extracellular solution during K(+) influx, and, notably, that fluid flow restored the decreased [K(+)] at the cell surface in a flow rate-dependent manner. These results support the “convection-regulation hypothesis” and define a novel interpretation of fluid flow-induced modulation of ion channels. Nature Publishing Group 2016-12-22 /pmc/articles/PMC5177964/ /pubmed/28004830 http://dx.doi.org/10.1038/srep39585 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Kim, Jae Gon Park, Sang Woong Byun, Doyoung Choi, Wahn Soo Sung, Dong Jun Shin, Kyung Chul Kim, Hyun-ji Leem, Young-Eun Kang, Jong-Sun Cho, Hana Kim, Bokyung Cho, Sung I Bae, Young Min Fluid flow facilitates inward rectifier K(+) current by convectively restoring [K(+)] at the cell membrane surface |
title | Fluid flow facilitates inward rectifier K(+) current by convectively restoring [K(+)] at the cell membrane surface |
title_full | Fluid flow facilitates inward rectifier K(+) current by convectively restoring [K(+)] at the cell membrane surface |
title_fullStr | Fluid flow facilitates inward rectifier K(+) current by convectively restoring [K(+)] at the cell membrane surface |
title_full_unstemmed | Fluid flow facilitates inward rectifier K(+) current by convectively restoring [K(+)] at the cell membrane surface |
title_short | Fluid flow facilitates inward rectifier K(+) current by convectively restoring [K(+)] at the cell membrane surface |
title_sort | fluid flow facilitates inward rectifier k(+) current by convectively restoring [k(+)] at the cell membrane surface |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5177964/ https://www.ncbi.nlm.nih.gov/pubmed/28004830 http://dx.doi.org/10.1038/srep39585 |
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