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Fusion of green fluorescent protein to the C-terminus of granulysin alters its intracellular localization in comparison to the native molecule
The engineering of green fluorescent protein (GFP) fusion constructs in order to visibly tag a protein of interest has become a commonly used cell biology technique. Although caveats to this approach are obvious, literature reports in which the chimeric molecule behaves differently than the native m...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2004
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC517947/ https://www.ncbi.nlm.nih.gov/pubmed/15361253 http://dx.doi.org/10.1186/1477-5751-3-2 |