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Drosophila microRNA-34 Impairs Axon Pruning of Mushroom Body γ Neurons by Downregulating the Expression of Ecdysone Receptor

MicroRNA-34 (miR-34) is crucial for preventing chronic large-scale neurite degeneration in the aged brain of Drosophila melanogaster. Here we investigated the role of miR-34 in two other types of large-scale axon degeneration in Drosophila: axotomy-induced axon degeneration in olfactory sensory neur...

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Autores principales: Lai, Yen-Wei, Chu, Sao-Yu, Wei, Jia-Yi, Cheng, Chu-Ya, Li, Jian-Chiuan, Chen, Po-Lin, Chen, Chun-Hong, Yu, Hung-Hsiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5180235/
https://www.ncbi.nlm.nih.gov/pubmed/28008974
http://dx.doi.org/10.1038/srep39141
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author Lai, Yen-Wei
Chu, Sao-Yu
Wei, Jia-Yi
Cheng, Chu-Ya
Li, Jian-Chiuan
Chen, Po-Lin
Chen, Chun-Hong
Yu, Hung-Hsiang
author_facet Lai, Yen-Wei
Chu, Sao-Yu
Wei, Jia-Yi
Cheng, Chu-Ya
Li, Jian-Chiuan
Chen, Po-Lin
Chen, Chun-Hong
Yu, Hung-Hsiang
author_sort Lai, Yen-Wei
collection PubMed
description MicroRNA-34 (miR-34) is crucial for preventing chronic large-scale neurite degeneration in the aged brain of Drosophila melanogaster. Here we investigated the role of miR-34 in two other types of large-scale axon degeneration in Drosophila: axotomy-induced axon degeneration in olfactory sensory neurons (OSNs) and developmentally related axon pruning in mushroom body (MB) neurons. Ectopically overexpressed miR-34 did not inhibit axon degeneration in OSNs following axotomy, whereas ectopically overexpressed miR-34 in differentiated MB neurons impaired γ axon pruning. Intriguingly, the miR-34-induced γ axon pruning defect resulted from downregulating the expression of ecdysone receptor B1 (EcR-B1) in differentiated MB γ neurons. Notably, the separate overexpression of EcR-B1 or a transforming growth factor- β receptor Baboon, whose activation can upregulate the EcR-B1 expression, in MB neurons rescued the miR-34-induced γ axon pruning phenotype. Future investigations of miR-34 targets that regulate the expression of EcR-B1 in MB γ neurons are warranted to elucidate pathways that regulate axon pruning, and to provide insight into mechanisms that control large-scale axon degeneration in the nervous system.
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spelling pubmed-51802352016-12-29 Drosophila microRNA-34 Impairs Axon Pruning of Mushroom Body γ Neurons by Downregulating the Expression of Ecdysone Receptor Lai, Yen-Wei Chu, Sao-Yu Wei, Jia-Yi Cheng, Chu-Ya Li, Jian-Chiuan Chen, Po-Lin Chen, Chun-Hong Yu, Hung-Hsiang Sci Rep Article MicroRNA-34 (miR-34) is crucial for preventing chronic large-scale neurite degeneration in the aged brain of Drosophila melanogaster. Here we investigated the role of miR-34 in two other types of large-scale axon degeneration in Drosophila: axotomy-induced axon degeneration in olfactory sensory neurons (OSNs) and developmentally related axon pruning in mushroom body (MB) neurons. Ectopically overexpressed miR-34 did not inhibit axon degeneration in OSNs following axotomy, whereas ectopically overexpressed miR-34 in differentiated MB neurons impaired γ axon pruning. Intriguingly, the miR-34-induced γ axon pruning defect resulted from downregulating the expression of ecdysone receptor B1 (EcR-B1) in differentiated MB γ neurons. Notably, the separate overexpression of EcR-B1 or a transforming growth factor- β receptor Baboon, whose activation can upregulate the EcR-B1 expression, in MB neurons rescued the miR-34-induced γ axon pruning phenotype. Future investigations of miR-34 targets that regulate the expression of EcR-B1 in MB γ neurons are warranted to elucidate pathways that regulate axon pruning, and to provide insight into mechanisms that control large-scale axon degeneration in the nervous system. Nature Publishing Group 2016-12-23 /pmc/articles/PMC5180235/ /pubmed/28008974 http://dx.doi.org/10.1038/srep39141 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lai, Yen-Wei
Chu, Sao-Yu
Wei, Jia-Yi
Cheng, Chu-Ya
Li, Jian-Chiuan
Chen, Po-Lin
Chen, Chun-Hong
Yu, Hung-Hsiang
Drosophila microRNA-34 Impairs Axon Pruning of Mushroom Body γ Neurons by Downregulating the Expression of Ecdysone Receptor
title Drosophila microRNA-34 Impairs Axon Pruning of Mushroom Body γ Neurons by Downregulating the Expression of Ecdysone Receptor
title_full Drosophila microRNA-34 Impairs Axon Pruning of Mushroom Body γ Neurons by Downregulating the Expression of Ecdysone Receptor
title_fullStr Drosophila microRNA-34 Impairs Axon Pruning of Mushroom Body γ Neurons by Downregulating the Expression of Ecdysone Receptor
title_full_unstemmed Drosophila microRNA-34 Impairs Axon Pruning of Mushroom Body γ Neurons by Downregulating the Expression of Ecdysone Receptor
title_short Drosophila microRNA-34 Impairs Axon Pruning of Mushroom Body γ Neurons by Downregulating the Expression of Ecdysone Receptor
title_sort drosophila microrna-34 impairs axon pruning of mushroom body γ neurons by downregulating the expression of ecdysone receptor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5180235/
https://www.ncbi.nlm.nih.gov/pubmed/28008974
http://dx.doi.org/10.1038/srep39141
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