Cargando…
AKR1B10 promotes breast cancer metastasis through integrin α5/δ-catenin mediated FAK/Src/Rac1 signaling pathway
Aldo-keto reductase 1B10 (AKR1B10) is not expressed in normal breast, but upregulated in primary and metastatic breast cancers, being a negative prognostic factor. This study characterized the molecular mechanisms of AKR1B10-promoted breast cancer metastasis. Ectopic expression of AKR1B10 in breast...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5190059/ https://www.ncbi.nlm.nih.gov/pubmed/27248472 http://dx.doi.org/10.18632/oncotarget.9672 |
_version_ | 1782487342874886144 |
---|---|
author | Huang, Chenfei Verhulst, Steven Shen, Yi Bu, Yiwen Cao, Yu He, Yingchun Wang, Yuhong Huang, Dan Cai, Chun Rao, Krishna Liao, Duan-Fang Jin, Junfei Cao, Deliang |
author_facet | Huang, Chenfei Verhulst, Steven Shen, Yi Bu, Yiwen Cao, Yu He, Yingchun Wang, Yuhong Huang, Dan Cai, Chun Rao, Krishna Liao, Duan-Fang Jin, Junfei Cao, Deliang |
author_sort | Huang, Chenfei |
collection | PubMed |
description | Aldo-keto reductase 1B10 (AKR1B10) is not expressed in normal breast, but upregulated in primary and metastatic breast cancers, being a negative prognostic factor. This study characterized the molecular mechanisms of AKR1B10-promoted breast cancer metastasis. Ectopic expression of AKR1B10 in breast cancer cells MCF-7 and MDA-MB-231 or siRNA-mediated silencing in BT-20 cells affected cell adhesion, migration and invasion in cell culture, and metastasis to the lung in the nude mice through upregulation of integrin α5 and δ-catenin. Silencing of integrin α5 or δ-catenin eradicated the cell adhesion and migration enhanced by AKR1B10, both of which acted synergistically. In these cells, the integrin α5 mediated focal adhesion kinase (FAK) signaling pathway was activated by AKR1B10, which, along with δ-catenin, stimulated Rac1-mediated cell migration and movement. In human primary and lymph node metastatic breast cancer, AKR1B10, integrin α5 and δ-catenin were correlatively upregulated with r=0.645 (p<0.0001) and r=0.796 (p<0.0001), respectively. These data suggest that AKR1B10 promotes breast cancer metastasis through activation of the integrin α5 and δ-catenin mediated FAK/Src/Rac1 signaling pathway. |
format | Online Article Text |
id | pubmed-5190059 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-51900592017-01-05 AKR1B10 promotes breast cancer metastasis through integrin α5/δ-catenin mediated FAK/Src/Rac1 signaling pathway Huang, Chenfei Verhulst, Steven Shen, Yi Bu, Yiwen Cao, Yu He, Yingchun Wang, Yuhong Huang, Dan Cai, Chun Rao, Krishna Liao, Duan-Fang Jin, Junfei Cao, Deliang Oncotarget Research Paper Aldo-keto reductase 1B10 (AKR1B10) is not expressed in normal breast, but upregulated in primary and metastatic breast cancers, being a negative prognostic factor. This study characterized the molecular mechanisms of AKR1B10-promoted breast cancer metastasis. Ectopic expression of AKR1B10 in breast cancer cells MCF-7 and MDA-MB-231 or siRNA-mediated silencing in BT-20 cells affected cell adhesion, migration and invasion in cell culture, and metastasis to the lung in the nude mice through upregulation of integrin α5 and δ-catenin. Silencing of integrin α5 or δ-catenin eradicated the cell adhesion and migration enhanced by AKR1B10, both of which acted synergistically. In these cells, the integrin α5 mediated focal adhesion kinase (FAK) signaling pathway was activated by AKR1B10, which, along with δ-catenin, stimulated Rac1-mediated cell migration and movement. In human primary and lymph node metastatic breast cancer, AKR1B10, integrin α5 and δ-catenin were correlatively upregulated with r=0.645 (p<0.0001) and r=0.796 (p<0.0001), respectively. These data suggest that AKR1B10 promotes breast cancer metastasis through activation of the integrin α5 and δ-catenin mediated FAK/Src/Rac1 signaling pathway. Impact Journals LLC 2016-05-27 /pmc/articles/PMC5190059/ /pubmed/27248472 http://dx.doi.org/10.18632/oncotarget.9672 Text en Copyright: © 2016 Huang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Huang, Chenfei Verhulst, Steven Shen, Yi Bu, Yiwen Cao, Yu He, Yingchun Wang, Yuhong Huang, Dan Cai, Chun Rao, Krishna Liao, Duan-Fang Jin, Junfei Cao, Deliang AKR1B10 promotes breast cancer metastasis through integrin α5/δ-catenin mediated FAK/Src/Rac1 signaling pathway |
title | AKR1B10 promotes breast cancer metastasis through integrin α5/δ-catenin mediated FAK/Src/Rac1 signaling pathway |
title_full | AKR1B10 promotes breast cancer metastasis through integrin α5/δ-catenin mediated FAK/Src/Rac1 signaling pathway |
title_fullStr | AKR1B10 promotes breast cancer metastasis through integrin α5/δ-catenin mediated FAK/Src/Rac1 signaling pathway |
title_full_unstemmed | AKR1B10 promotes breast cancer metastasis through integrin α5/δ-catenin mediated FAK/Src/Rac1 signaling pathway |
title_short | AKR1B10 promotes breast cancer metastasis through integrin α5/δ-catenin mediated FAK/Src/Rac1 signaling pathway |
title_sort | akr1b10 promotes breast cancer metastasis through integrin α5/δ-catenin mediated fak/src/rac1 signaling pathway |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5190059/ https://www.ncbi.nlm.nih.gov/pubmed/27248472 http://dx.doi.org/10.18632/oncotarget.9672 |
work_keys_str_mv | AT huangchenfei akr1b10promotesbreastcancermetastasisthroughintegrina5dcateninmediatedfaksrcrac1signalingpathway AT verhulststeven akr1b10promotesbreastcancermetastasisthroughintegrina5dcateninmediatedfaksrcrac1signalingpathway AT shenyi akr1b10promotesbreastcancermetastasisthroughintegrina5dcateninmediatedfaksrcrac1signalingpathway AT buyiwen akr1b10promotesbreastcancermetastasisthroughintegrina5dcateninmediatedfaksrcrac1signalingpathway AT caoyu akr1b10promotesbreastcancermetastasisthroughintegrina5dcateninmediatedfaksrcrac1signalingpathway AT heyingchun akr1b10promotesbreastcancermetastasisthroughintegrina5dcateninmediatedfaksrcrac1signalingpathway AT wangyuhong akr1b10promotesbreastcancermetastasisthroughintegrina5dcateninmediatedfaksrcrac1signalingpathway AT huangdan akr1b10promotesbreastcancermetastasisthroughintegrina5dcateninmediatedfaksrcrac1signalingpathway AT caichun akr1b10promotesbreastcancermetastasisthroughintegrina5dcateninmediatedfaksrcrac1signalingpathway AT raokrishna akr1b10promotesbreastcancermetastasisthroughintegrina5dcateninmediatedfaksrcrac1signalingpathway AT liaoduanfang akr1b10promotesbreastcancermetastasisthroughintegrina5dcateninmediatedfaksrcrac1signalingpathway AT jinjunfei akr1b10promotesbreastcancermetastasisthroughintegrina5dcateninmediatedfaksrcrac1signalingpathway AT caodeliang akr1b10promotesbreastcancermetastasisthroughintegrina5dcateninmediatedfaksrcrac1signalingpathway |