Silencing DNA methyltransferase 1 (DNMT1) inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK

Our previous study showed DNMT1 is up-regulated in esophageal squamous cell carcinoma (ESCC), which is associated with methylation of tumor suppressors. In the current study, we investigate the role of DNMT1 in ESCC. We found silencing DNMT1 inhibited proliferation, metastasis and invasion of three...

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Autores principales: Bai, Jian, Zhang, Xue, Hu, Kai, Liu, Bangqing, Wang, Haiyong, Li, Angui, Lin, Feng, Zhang, Lifei, Sun, Xiaolin, Du, Zhenzong, Song, Jianfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5190084/
https://www.ncbi.nlm.nih.gov/pubmed/27286455
http://dx.doi.org/10.18632/oncotarget.9866
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author Bai, Jian
Zhang, Xue
Hu, Kai
Liu, Bangqing
Wang, Haiyong
Li, Angui
Lin, Feng
Zhang, Lifei
Sun, Xiaolin
Du, Zhenzong
Song, Jianfei
author_facet Bai, Jian
Zhang, Xue
Hu, Kai
Liu, Bangqing
Wang, Haiyong
Li, Angui
Lin, Feng
Zhang, Lifei
Sun, Xiaolin
Du, Zhenzong
Song, Jianfei
author_sort Bai, Jian
collection PubMed
description Our previous study showed DNMT1 is up-regulated in esophageal squamous cell carcinoma (ESCC), which is associated with methylation of tumor suppressors. In the current study, we investigate the role of DNMT1 in ESCC. We found silencing DNMT1 inhibited proliferation, metastasis and invasion of three different ESCC cells, K150, K410 and K450. We also found silencing DNMT1 induced G1 arrest and cell apoptosis in K150, K410 and K450 cells. In vivo study showed silencing DNMT1 suppressed tumor growth in nude mice. In addition, silencing DNMT1 increased expression of tumor suppressor genes, RASSF1A and DAPK, in ESCC cells and ESCC xenograft in nude mice. Moreover, silencing DNMT1 decreased methylation in promoter of RASSF1A and DAPK. In conclusion, our data demonstrated that silencing DNMT1 inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK.
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spelling pubmed-51900842017-01-05 Silencing DNA methyltransferase 1 (DNMT1) inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK Bai, Jian Zhang, Xue Hu, Kai Liu, Bangqing Wang, Haiyong Li, Angui Lin, Feng Zhang, Lifei Sun, Xiaolin Du, Zhenzong Song, Jianfei Oncotarget Research Paper Our previous study showed DNMT1 is up-regulated in esophageal squamous cell carcinoma (ESCC), which is associated with methylation of tumor suppressors. In the current study, we investigate the role of DNMT1 in ESCC. We found silencing DNMT1 inhibited proliferation, metastasis and invasion of three different ESCC cells, K150, K410 and K450. We also found silencing DNMT1 induced G1 arrest and cell apoptosis in K150, K410 and K450 cells. In vivo study showed silencing DNMT1 suppressed tumor growth in nude mice. In addition, silencing DNMT1 increased expression of tumor suppressor genes, RASSF1A and DAPK, in ESCC cells and ESCC xenograft in nude mice. Moreover, silencing DNMT1 decreased methylation in promoter of RASSF1A and DAPK. In conclusion, our data demonstrated that silencing DNMT1 inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK. Impact Journals LLC 2016-06-07 /pmc/articles/PMC5190084/ /pubmed/27286455 http://dx.doi.org/10.18632/oncotarget.9866 Text en Copyright: © 2016 Bai et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Bai, Jian
Zhang, Xue
Hu, Kai
Liu, Bangqing
Wang, Haiyong
Li, Angui
Lin, Feng
Zhang, Lifei
Sun, Xiaolin
Du, Zhenzong
Song, Jianfei
Silencing DNA methyltransferase 1 (DNMT1) inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK
title Silencing DNA methyltransferase 1 (DNMT1) inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK
title_full Silencing DNA methyltransferase 1 (DNMT1) inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK
title_fullStr Silencing DNA methyltransferase 1 (DNMT1) inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK
title_full_unstemmed Silencing DNA methyltransferase 1 (DNMT1) inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK
title_short Silencing DNA methyltransferase 1 (DNMT1) inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK
title_sort silencing dna methyltransferase 1 (dnmt1) inhibits proliferation, metastasis and invasion in escc by suppressing methylation of rassf1a and dapk
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5190084/
https://www.ncbi.nlm.nih.gov/pubmed/27286455
http://dx.doi.org/10.18632/oncotarget.9866
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