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Critical Function of PRDM2 in the Neoplastic Growth of Testicular Germ Cell Tumors
Testicular germ cell tumors (TGCTs) derive from primordial germ cells. Their maturation is blocked at different stages, reflecting histological tumor subtypes. A common genetic alteration in TGCT is a deletion of the chromosome 1 short arm, where the PRDM2 gene, belonging to the Positive Regulatory...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192434/ https://www.ncbi.nlm.nih.gov/pubmed/27983647 http://dx.doi.org/10.3390/biology5040054 |
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author | Di Zazzo, Erika Porcile, Carola Bartollino, Silvia Moncharmont, Bruno |
author_facet | Di Zazzo, Erika Porcile, Carola Bartollino, Silvia Moncharmont, Bruno |
author_sort | Di Zazzo, Erika |
collection | PubMed |
description | Testicular germ cell tumors (TGCTs) derive from primordial germ cells. Their maturation is blocked at different stages, reflecting histological tumor subtypes. A common genetic alteration in TGCT is a deletion of the chromosome 1 short arm, where the PRDM2 gene, belonging to the Positive Regulatory domain gene (PRDM) family, is located. Expression of PRDM2 gene is shifted in different human tumors, where the expression of the two principal protein forms coded by PRDM2 gene, RIZ1 and RIZ2, is frequently unbalanced. Therefore, PRDM2 is actually considered a candidate tumor suppressor gene in different types of cancer. Although recent studies have demonstrated that PRDM gene family members have a pivotal role during the early stages of testicular development, no information are actually available on the involvement of these genes in TGCTs. In this article we show by qRT-PCR analysis that PRDM2 expression level is modulated by proliferation and differentiation agents such as estradiol, whose exposure during fetal life is probably an important risk factor for TGCTs development in adulthood. Furthermore in normal and cancer germ cell lines, PRDM2 binds estradiol receptor α (ERα) and influences proliferation, survival and apoptosis, as previously reported using MCF-7 breast cancer cell line, suggesting a potential tumor-suppressor role in TGCT formation. |
format | Online Article Text |
id | pubmed-5192434 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-51924342017-01-03 Critical Function of PRDM2 in the Neoplastic Growth of Testicular Germ Cell Tumors Di Zazzo, Erika Porcile, Carola Bartollino, Silvia Moncharmont, Bruno Biology (Basel) Article Testicular germ cell tumors (TGCTs) derive from primordial germ cells. Their maturation is blocked at different stages, reflecting histological tumor subtypes. A common genetic alteration in TGCT is a deletion of the chromosome 1 short arm, where the PRDM2 gene, belonging to the Positive Regulatory domain gene (PRDM) family, is located. Expression of PRDM2 gene is shifted in different human tumors, where the expression of the two principal protein forms coded by PRDM2 gene, RIZ1 and RIZ2, is frequently unbalanced. Therefore, PRDM2 is actually considered a candidate tumor suppressor gene in different types of cancer. Although recent studies have demonstrated that PRDM gene family members have a pivotal role during the early stages of testicular development, no information are actually available on the involvement of these genes in TGCTs. In this article we show by qRT-PCR analysis that PRDM2 expression level is modulated by proliferation and differentiation agents such as estradiol, whose exposure during fetal life is probably an important risk factor for TGCTs development in adulthood. Furthermore in normal and cancer germ cell lines, PRDM2 binds estradiol receptor α (ERα) and influences proliferation, survival and apoptosis, as previously reported using MCF-7 breast cancer cell line, suggesting a potential tumor-suppressor role in TGCT formation. MDPI 2016-12-14 /pmc/articles/PMC5192434/ /pubmed/27983647 http://dx.doi.org/10.3390/biology5040054 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Di Zazzo, Erika Porcile, Carola Bartollino, Silvia Moncharmont, Bruno Critical Function of PRDM2 in the Neoplastic Growth of Testicular Germ Cell Tumors |
title | Critical Function of PRDM2 in the Neoplastic Growth of Testicular Germ Cell Tumors |
title_full | Critical Function of PRDM2 in the Neoplastic Growth of Testicular Germ Cell Tumors |
title_fullStr | Critical Function of PRDM2 in the Neoplastic Growth of Testicular Germ Cell Tumors |
title_full_unstemmed | Critical Function of PRDM2 in the Neoplastic Growth of Testicular Germ Cell Tumors |
title_short | Critical Function of PRDM2 in the Neoplastic Growth of Testicular Germ Cell Tumors |
title_sort | critical function of prdm2 in the neoplastic growth of testicular germ cell tumors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192434/ https://www.ncbi.nlm.nih.gov/pubmed/27983647 http://dx.doi.org/10.3390/biology5040054 |
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