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Oxidized epigallocatechin gallate inhibited lysozyme fibrillation more strongly than the native form
Epigallocatechin gallate (EGCG), the most abundant flavanoid in green tea, is currently being evaluated in the clinic due to its benefits in the treatment of amyloid disorders. Its anti-amyloidogenic effect has been attributed to direct interaction of the intact molecule with misfolded polypeptides....
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5199191/ https://www.ncbi.nlm.nih.gov/pubmed/28038426 http://dx.doi.org/10.1016/j.redox.2016.12.016 |
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author | An, Ting-Ting Feng, Shuang Zeng, Cheng-Ming |
author_facet | An, Ting-Ting Feng, Shuang Zeng, Cheng-Ming |
author_sort | An, Ting-Ting |
collection | PubMed |
description | Epigallocatechin gallate (EGCG), the most abundant flavanoid in green tea, is currently being evaluated in the clinic due to its benefits in the treatment of amyloid disorders. Its anti-amyloidogenic effect has been attributed to direct interaction of the intact molecule with misfolded polypeptides. In addition, antioxidant activity is also involved in the anti-amyloidogenic role. The detailed molecular mechanism is still unclear and requires further investigation. In the present study, the kinetics of EGCG oxidation and the anti-amyloidogenic effect of the resultant oxidation substances have been examined. The results indicate that EGCG degrades in a medium at pH 8.0 with a half-life less than 2 h. By utilizing lysozyme as an in vitro model, the oxidized EGCG demonstrates a more potent anti-amyloidogenic capacity than the intact molecule, as shown by ThT and ANS fluorescence, TEM determination, and hemolytic assay. The oxidized EGCG also has a stronger disruptive effect on preformed fibrils than the native form. Ascorbic acid eliminates the disruptive role of native EGCG on the fibrils, suggesting that oxidation is a prerequisite in fibril disruption. The results of this work demonstrate that oxidized EGCG plays a more important role than the intact molecule in anti-amyloidogenic activity. These insights into the action of EGCG may provide a novel route to understand the anti-amyloidogenic activity of natural polyphenols. |
format | Online Article Text |
id | pubmed-5199191 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-51991912017-01-06 Oxidized epigallocatechin gallate inhibited lysozyme fibrillation more strongly than the native form An, Ting-Ting Feng, Shuang Zeng, Cheng-Ming Redox Biol Research Paper Epigallocatechin gallate (EGCG), the most abundant flavanoid in green tea, is currently being evaluated in the clinic due to its benefits in the treatment of amyloid disorders. Its anti-amyloidogenic effect has been attributed to direct interaction of the intact molecule with misfolded polypeptides. In addition, antioxidant activity is also involved in the anti-amyloidogenic role. The detailed molecular mechanism is still unclear and requires further investigation. In the present study, the kinetics of EGCG oxidation and the anti-amyloidogenic effect of the resultant oxidation substances have been examined. The results indicate that EGCG degrades in a medium at pH 8.0 with a half-life less than 2 h. By utilizing lysozyme as an in vitro model, the oxidized EGCG demonstrates a more potent anti-amyloidogenic capacity than the intact molecule, as shown by ThT and ANS fluorescence, TEM determination, and hemolytic assay. The oxidized EGCG also has a stronger disruptive effect on preformed fibrils than the native form. Ascorbic acid eliminates the disruptive role of native EGCG on the fibrils, suggesting that oxidation is a prerequisite in fibril disruption. The results of this work demonstrate that oxidized EGCG plays a more important role than the intact molecule in anti-amyloidogenic activity. These insights into the action of EGCG may provide a novel route to understand the anti-amyloidogenic activity of natural polyphenols. Elsevier 2016-12-21 /pmc/articles/PMC5199191/ /pubmed/28038426 http://dx.doi.org/10.1016/j.redox.2016.12.016 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Paper An, Ting-Ting Feng, Shuang Zeng, Cheng-Ming Oxidized epigallocatechin gallate inhibited lysozyme fibrillation more strongly than the native form |
title | Oxidized epigallocatechin gallate inhibited lysozyme fibrillation more strongly than the native form |
title_full | Oxidized epigallocatechin gallate inhibited lysozyme fibrillation more strongly than the native form |
title_fullStr | Oxidized epigallocatechin gallate inhibited lysozyme fibrillation more strongly than the native form |
title_full_unstemmed | Oxidized epigallocatechin gallate inhibited lysozyme fibrillation more strongly than the native form |
title_short | Oxidized epigallocatechin gallate inhibited lysozyme fibrillation more strongly than the native form |
title_sort | oxidized epigallocatechin gallate inhibited lysozyme fibrillation more strongly than the native form |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5199191/ https://www.ncbi.nlm.nih.gov/pubmed/28038426 http://dx.doi.org/10.1016/j.redox.2016.12.016 |
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