Partial Purification of a Megadalton DNA Replication Complex by Free Flow Electrophoresis

We describe a gentle and rapid method to purify the intact multiprotein DNA replication complex using free flow electrophoresis (FFE). In particular, we applied FFE to purify the human cell DNA synthesome, which is a multiprotein complex that is fully competent to carry-out all phases of the DNA rep...

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Detalles Bibliográficos
Autores principales: Li, Caroline M., Miao, Yunan, Lingeman, Robert G., Hickey, Robert J., Malkas, Linda H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5201288/
https://www.ncbi.nlm.nih.gov/pubmed/28036377
http://dx.doi.org/10.1371/journal.pone.0169259

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5201288/
https://www.ncbi.nlm.nih.gov/pubmed/28036377
http://dx.doi.org/10.1371/journal.pone.0169259

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