Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts

KEY MESSAGE: Altered starch quality with full knockout of GBSS gene function in potato was achieved using CRISPR-Cas9 technology, through transient transfection and regeneration from isolated protoplasts. ABSTRACT: Site-directed mutagenesis (SDM) has shown great progress in introducing precisely tar...

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Detalles Bibliográficos
Autores principales: Andersson, Mariette, Turesson, Helle, Nicolia, Alessandro, Fält, Ann-Sofie, Samuelsson, Mathias, Hofvander, Per
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5206254/
https://www.ncbi.nlm.nih.gov/pubmed/27699473
http://dx.doi.org/10.1007/s00299-016-2062-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5206254/
https://www.ncbi.nlm.nih.gov/pubmed/27699473
http://dx.doi.org/10.1007/s00299-016-2062-3

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