Cargando…

Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts

KEY MESSAGE: Altered starch quality with full knockout of GBSS gene function in potato was achieved using CRISPR-Cas9 technology, through transient transfection and regeneration from isolated protoplasts. ABSTRACT: Site-directed mutagenesis (SDM) has shown great progress in introducing precisely tar...

Descripción completa

Detalles Bibliográficos
Autores principales:	Andersson, Mariette, Turesson, Helle, Nicolia, Alessandro, Fält, Ann-Sofie, Samuelsson, Mathias, Hofvander, Per
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2016
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5206254/ https://www.ncbi.nlm.nih.gov/pubmed/27699473 http://dx.doi.org/10.1007/s00299-016-2062-3

Ejemplares similares

Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato
por: Zhao, Xue, et al.
Publicado: (2021)

Establishment of a DNA-free genome editing and protoplast regeneration method in cultivated tomato (Solanum lycopersicum)
por: Liu, Ying, et al.
Publicado: (2022)

Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato
por: Andersson, Mariette, et al.
Publicado: (2018)

Pho1a (plastid starch phosphorylase) is duplicated and essential for normal starch granule phenotype in tubers of Solanum tuberosum L
por: Sharma, Shrikant, et al.
Publicado: (2023)

Genomic Selection for Late Blight and Common Scab Resistance in Tetraploid Potato (Solanum tuberosum)
por: Enciso-Rodriguez, Felix, et al.
Publicado: (2018)

Mapping and QTL Analysis of Early-Maturity Traits in Tetraploid Potato (Solanum tuberosum L.)
por: Li, Xingcui, et al.
Publicado: (2018)

Reduced Enzymatic Browning in Potato Tubers by Specific Editing of a Polyphenol Oxidase Gene via Ribonucleoprotein Complexes Delivery of the CRISPR/Cas9 System
por: González, Matías Nicolás, et al.
Publicado: (2020)

Genetic Linkage Mapping of Economically Important Traits in Cultivated Tetraploid Potato (Solanum tuberosum L.)
por: Massa, Alicia N., et al.
Publicado: (2015)

Tetraploid somatic hybrids of potato (Solanum tuberosum L.) obtained from diploid breeding lines
por: Przetakiewicz, Jarosław, et al.
Publicado: (2006)

Sampling Variation of RAD-Seq Data from Diploid and Tetraploid Potato (Solanum tuberosum L.)
por: Dang, Zhenyu, et al.
Publicado: (2021)

Regenerative plantlets with improved agronomic characteristics caused by anther culture of tetraploid potato (Solanum tuberosum L.)
por: Zhang, Li, et al.
Publicado: (2023)

Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (Solanum tuberosum L.)
por: Schumacher, Christina, et al.
Publicado: (2021)

QTL analysis reveals quantitative resistant loci for Phytophthora infestans and Tecia solanivora in tetraploid potato (Solanum tuberosum L.)
por: Santa, Juan David, et al.
Publicado: (2018)

Identification of Long-Distance Transport Signal Molecules Associated with Plant Maturity in Tetraploid Cultivated Potatoes (Solanum tuberosum L.)
por: Hui, Zhiming, et al.
Publicado: (2022)

Natural diversity of potato (Solanum tuberosum) invertases
por: Draffehn, Astrid M, et al.
Publicado: (2010)

DNA-free CRISPR-Cas9 gene editing of wild tetraploid tomato Solanum peruvianum using protoplast regeneration
por: Lin, Choun-Sea, et al.
Publicado: (2022)

Strategies for Efficient Gene Editing in Protoplasts of Solanum tuberosum Theme: Determining gRNA Efficiency Design by Utilizing Protoplast (Research)
por: Carlsen, Frida Meijer, et al.
Publicado: (2022)

Impact of Globodera ellingtonae on yield of potato (Solanum tuberosum)
por: Zasada, Inga A., et al.
Publicado: (2019)

32Starch metabolism in potato Solanum tuberosum L.
por: Sergeeva, E.M., et al.
Publicado: (2022)

Biofungicides for Improvement of Potato (Solanum tuberosum L) Production
por: Ehiobu, John, et al.
Publicado: (2022)

Generation and Inheritance of Targeted Mutations in Potato (Solanum tuberosum L.) Using the CRISPR/Cas System
por: Butler, Nathaniel M., et al.
Publicado: (2015)

Genome-Based Genotype × Environment Prediction Enhances Potato (Solanum tuberosum L.) Improvement Using Pseudo-Diploid and Polysomic Tetraploid Modeling
por: Ortiz, Rodomiro, et al.
Publicado: (2022)

Corrigendum: Strategies for Efficient Gene Editing in Protoplasts of Solanum tuberosum Theme: Determining gRNA Efficiency Design by Utilizing Protoplast (Research)
por: Carlsen, Frida Meijer, et al.
Publicado: (2022)

Potato tuber expression of Arabidopsis WRINKLED1 increase triacylglycerol and membrane lipids while affecting central carbohydrate metabolism
por: Hofvander, Per, et al.
Publicado: (2016)

Optimizing TILLING and Ecotilling techniques for potato (Solanum tuberosum L)
por: Elias, Rana, et al.
Publicado: (2009)

Prediction based on estimated breeding values using genealogy for tuber yield and late blight resistance in auto-tetraploid potato (Solanum tuberosum L.)
por: Sood, Salej, et al.
Publicado: (2020)

Involvement of Potato (Solanum tuberosum L.) MKK6 in Response to Potato virus Y
por: Lazar, Ana, et al.
Publicado: (2014)

Multiple alleles for resistance and susceptibility modulate the defense response in the interaction of tetraploid potato (Solanum tuberosum) with Synchytrium endobioticum pathotypes 1, 2, 6 and 18
por: Ballvora, Agim, et al.
Publicado: (2011)

Genotype-Specific Differences in Phosphorus Efficiency of Potato (Solanum tuberosum L.)
por: Wacker-Fester, Katrin, et al.
Publicado: (2019)

Solyntus, the New Highly Contiguous Reference Genome for Potato (Solanum tuberosum)
por: van Lieshout, Natascha, et al.
Publicado: (2020)

Cultivar-specific nutritional status of potato (Solanum tuberosum L.) crops
por: Coulibali, Zonlehoua, et al.
Publicado: (2020)

Natural resistance to Potato virus Y in Solanum tuberosum Group Phureja
por: Torrance, Lesley, et al.
Publicado: (2020)

The impact of haulm killing on yield and quality of potato (Solanum tuberosum L.)
por: Karan, Yasin Bedrettin
Publicado: (2021)

The complete mitochondrial genome sequences of potato (Solanum tuberosum L., Solanaceae)
por: Cho, Kwang-Soo, et al.
Publicado: (2017)

Study on the cultivation of seedlings using buds of potato (Solanum tuberosum L.)
por: Wang, Chaonan, et al.
Publicado: (2022)

Chloroplast genome sequence of the wild tetraploid potato relative Solanum stoloniferum
por: Park, Tae-Ho
Publicado: (2018)

Evaluation of antioxidant activity of three common potato (Solanum tuberosum) cultivars in Iran
por: Hesam, Faride, et al.
Publicado: (2012)

Cereal cystatins delay sprouting and nutrient loss in tubers of potato, Solanum tuberosum
por: Munger, Aurélie, et al.
Publicado: (2015)

Genome-wide analysis of starch metabolism genes in potato (Solanum tuberosum L.)
por: Van Harsselaar, Jessica K., et al.
Publicado: (2017)

Transcriptome Profiling and Characterization of Drought-Tolerant Potato Plant (Solanum tuberosum L.)
por: Moon, Ki-Beom, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_ae566ikuniku74a2l0tt3c098i