Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries

BACKGROUND: We developed a method to make a various high quality random peptide libraries for evolutionary protein engineering based on a combinatorial DNA synthesis. RESULTS: A split synthesis in codon units was performed with mixtures of bases optimally designed by using a Genetic Algorithm progra...

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Detalles Bibliográficos
Autores principales: Tabuchi, Ichiro, Soramoto, Sayaka, Ueno, Shingo, Husimi, Yuzuru
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC520752/
https://www.ncbi.nlm.nih.gov/pubmed/15341664
http://dx.doi.org/10.1186/1472-6750-4-19

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC520752/
https://www.ncbi.nlm.nih.gov/pubmed/15341664
http://dx.doi.org/10.1186/1472-6750-4-19

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