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Using the cre-lox recombination system to assess functional impairment caused by amino acid substitutions in yeast proteins
A method was developed to assess the functional significance of a sequence motif in yeast Upf3p, a protein required for nonsense-mediated mRNA decay (NMD). The motif lies at the edge of the Upf3p-Upf2p interaction domain, but at the same time resembles the canonical leucine-rich nuclear export seque...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Biological Procedures Online
2004
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC521343/ https://www.ncbi.nlm.nih.gov/pubmed/15472721 http://dx.doi.org/10.1251/bpo91 |