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Microscale Measurements of Michaelis–Menten Constants of Neuraminidase with Nanogel Capillary Electrophoresis for the Determination of the Sialic Acid Linkage

[Image: see text] Phospholipid nanogels enhance the stability and performance of the exoglycosidase enzyme neuraminidase and are used to create a fixed zone of enzyme within a capillary. With nanogels, there is no need to covalently immobilize the enzyme, as it is physically constrained. This enable...

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Detalles Bibliográficos
Autores principales: Gattu, Srikanth, Crihfield, Cassandra. L., Holland, Lisa A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2016
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5214287/
https://www.ncbi.nlm.nih.gov/pubmed/27936604
http://dx.doi.org/10.1021/acs.analchem.6b04074