Insertional Mutagenesis by CRISPR/Cas9 Ribonucleoprotein Gene Editing in Cells Targeted for Point Mutation Repair Directed by Short Single-Stranded DNA Oligonucleotides

CRISPR/Cas9 and single-stranded DNA oligonucleotides (ssODNs) have been used to direct the repair of a single base mutation in human genes. Here, we examine a method designed to increase the precision of RNA guided genome editing in human cells by utilizing a CRISPR/Cas9 ribonucleoprotein (RNP) comp...

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Detalles Bibliográficos
Autores principales: Rivera-Torres, Natalia, Banas, Kelly, Bialk, Pawel, Bloh, Kevin M., Kmiec, Eric B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5214427/
https://www.ncbi.nlm.nih.gov/pubmed/28052104
http://dx.doi.org/10.1371/journal.pone.0169350

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5214427/
https://www.ncbi.nlm.nih.gov/pubmed/28052104
http://dx.doi.org/10.1371/journal.pone.0169350

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