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Insertional Mutagenesis by CRISPR/Cas9 Ribonucleoprotein Gene Editing in Cells Targeted for Point Mutation Repair Directed by Short Single-Stranded DNA Oligonucleotides

CRISPR/Cas9 and single-stranded DNA oligonucleotides (ssODNs) have been used to direct the repair of a single base mutation in human genes. Here, we examine a method designed to increase the precision of RNA guided genome editing in human cells by utilizing a CRISPR/Cas9 ribonucleoprotein (RNP) comp...

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Detalles Bibliográficos
Autores principales:	Rivera-Torres, Natalia, Banas, Kelly, Bialk, Pawel, Bloh, Kevin M., Kmiec, Eric B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5214427/ https://www.ncbi.nlm.nih.gov/pubmed/28052104 http://dx.doi.org/10.1371/journal.pone.0169350

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