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TRIM32-Cytoplasmic-Body Formation Is an ATP-Consuming Process Stimulated by HSP70 in Cells

The spontaneous and energy-releasing reaction of protein aggregation is typically prevented by cellular quality control machinery (QC). TRIM32 is a member of the TRIM (tripartite motif-containing) ubiquitin E3 ligases, and when overexpressed in cultured cells, readily forms spherical inclusions desi...

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Autores principales: Kawaguchi, Yuki, Taoka, Masato, Takekiyo, Takahiro, Uekita, Takamasa, Shoji, Ikuo, Hachiya, Naomi, Ichimura, Tohru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5215751/
https://www.ncbi.nlm.nih.gov/pubmed/28052117
http://dx.doi.org/10.1371/journal.pone.0169436
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author Kawaguchi, Yuki
Taoka, Masato
Takekiyo, Takahiro
Uekita, Takamasa
Shoji, Ikuo
Hachiya, Naomi
Ichimura, Tohru
author_facet Kawaguchi, Yuki
Taoka, Masato
Takekiyo, Takahiro
Uekita, Takamasa
Shoji, Ikuo
Hachiya, Naomi
Ichimura, Tohru
author_sort Kawaguchi, Yuki
collection PubMed
description The spontaneous and energy-releasing reaction of protein aggregation is typically prevented by cellular quality control machinery (QC). TRIM32 is a member of the TRIM (tripartite motif-containing) ubiquitin E3 ligases, and when overexpressed in cultured cells, readily forms spherical inclusions designated as cytoplasmic bodies (CBs) even without proteasome inhibition. Here, we show that HSP70, a central QC component, is a primary binding factor of overexpressed TRIM32. Contrary to expectation, however, we find that this molecular chaperone facilitates and stabilizes CB assembly depending on intrinsic ATPase activity, rather than preventing CB formation. We also show that the HSP70-TRIM32 complex is biochemically distinct from the previously characterized 14-3-3-TRIM32 phospho-complex. Moreover, the two complexes have opposing roles, with HSP70 stimulating CB formation and 14-3-3 retaining TRIM32 in a diffuse form throughout the cytosol. Our results suggest that CB inclusion formation is actively controlled by cellular QC and requires ATP, similar to protein folding and degradation reactions.
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spelling pubmed-52157512017-01-19 TRIM32-Cytoplasmic-Body Formation Is an ATP-Consuming Process Stimulated by HSP70 in Cells Kawaguchi, Yuki Taoka, Masato Takekiyo, Takahiro Uekita, Takamasa Shoji, Ikuo Hachiya, Naomi Ichimura, Tohru PLoS One Research Article The spontaneous and energy-releasing reaction of protein aggregation is typically prevented by cellular quality control machinery (QC). TRIM32 is a member of the TRIM (tripartite motif-containing) ubiquitin E3 ligases, and when overexpressed in cultured cells, readily forms spherical inclusions designated as cytoplasmic bodies (CBs) even without proteasome inhibition. Here, we show that HSP70, a central QC component, is a primary binding factor of overexpressed TRIM32. Contrary to expectation, however, we find that this molecular chaperone facilitates and stabilizes CB assembly depending on intrinsic ATPase activity, rather than preventing CB formation. We also show that the HSP70-TRIM32 complex is biochemically distinct from the previously characterized 14-3-3-TRIM32 phospho-complex. Moreover, the two complexes have opposing roles, with HSP70 stimulating CB formation and 14-3-3 retaining TRIM32 in a diffuse form throughout the cytosol. Our results suggest that CB inclusion formation is actively controlled by cellular QC and requires ATP, similar to protein folding and degradation reactions. Public Library of Science 2017-01-04 /pmc/articles/PMC5215751/ /pubmed/28052117 http://dx.doi.org/10.1371/journal.pone.0169436 Text en © 2017 Kawaguchi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kawaguchi, Yuki
Taoka, Masato
Takekiyo, Takahiro
Uekita, Takamasa
Shoji, Ikuo
Hachiya, Naomi
Ichimura, Tohru
TRIM32-Cytoplasmic-Body Formation Is an ATP-Consuming Process Stimulated by HSP70 in Cells
title TRIM32-Cytoplasmic-Body Formation Is an ATP-Consuming Process Stimulated by HSP70 in Cells
title_full TRIM32-Cytoplasmic-Body Formation Is an ATP-Consuming Process Stimulated by HSP70 in Cells
title_fullStr TRIM32-Cytoplasmic-Body Formation Is an ATP-Consuming Process Stimulated by HSP70 in Cells
title_full_unstemmed TRIM32-Cytoplasmic-Body Formation Is an ATP-Consuming Process Stimulated by HSP70 in Cells
title_short TRIM32-Cytoplasmic-Body Formation Is an ATP-Consuming Process Stimulated by HSP70 in Cells
title_sort trim32-cytoplasmic-body formation is an atp-consuming process stimulated by hsp70 in cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5215751/
https://www.ncbi.nlm.nih.gov/pubmed/28052117
http://dx.doi.org/10.1371/journal.pone.0169436
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