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Using Next-Generation Sequencing for DNA Barcoding: Capturing Allelic Variation in ITS2

Internal Transcribed Spacer 2 (ITS2) is a popular DNA barcoding marker; however, in some animal species it is hypervariable and therefore difficult to sequence with traditional methods. With next-generation sequencing (NGS) it is possible to sequence all gene variants despite the presence of single...

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Detalles Bibliográficos
Autores principales: Batovska, Jana, Cogan, Noel O. I., Lynch, Stacey E., Blacket, Mark J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5217108/
https://www.ncbi.nlm.nih.gov/pubmed/27799340
http://dx.doi.org/10.1534/g3.116.036145