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Development of a SYBR Green I real-time PCR for the detection of the orf virus

Orf is a non-systemic, ubiquitous disease of sheep and goats caused by the orf virus (ORFV). ORFV occasionally causes cutaneous lesions in humans in contact with infected animals. In the present study, a real-time PCR method was established for detection of ORFV using the fluorescent chimeric dye SY...

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Detalles Bibliográficos
Autores principales: Wang, Yong, Yang, Kankan, Bai, Caixia, Yin, Dongdong, Li, Gang, Qi, Kezong, Wang, Guijun, Li, Yongdong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5218949/
https://www.ncbi.nlm.nih.gov/pubmed/28063148
http://dx.doi.org/10.1186/s13568-016-0322-9