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A novel method to accurately locate and count large numbers of steps by photobleaching

Photobleaching event counting is a single-molecule fluorescence technique that is increasingly being used to determine the stoichiometry of protein and RNA complexes composed of many subunits in vivo as well as in vitro. By tagging protein or RNA subunits with fluorophores, activating them, and subs...

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Detalles Bibliográficos
Autores principales: Tsekouras, Konstantinos, Custer, Thomas C., Jashnsaz, Hossein, Walter, Nils G., Pressé, Steve
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221592/
https://www.ncbi.nlm.nih.gov/pubmed/27654946
http://dx.doi.org/10.1091/mbc.E16-06-0404