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The miR-34a-5p promotes the multi-chemoresistance of osteosarcoma via repression of the AGTR1 gene

BACKGROUND: Chemoresistance hinders the curative cancer chemotherapy. MicroRNAs (miRNAs) are key players in diverse biological processes including the chemoresistance of cancers. METHODS: A RNA-seq-based miR-omic analysis of osteosarcoma (OS) cells was performed to detect the levels of miR-34a-5p. B...

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Autores principales: Pu, Youguang, Zhao, Fangfang, Li, Yinpeng, Cui, Mingda, Wang, Haiyan, Meng, Xianghui, Cai, Shanbao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223322/
https://www.ncbi.nlm.nih.gov/pubmed/28073349
http://dx.doi.org/10.1186/s12885-016-3002-x
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author Pu, Youguang
Zhao, Fangfang
Li, Yinpeng
Cui, Mingda
Wang, Haiyan
Meng, Xianghui
Cai, Shanbao
author_facet Pu, Youguang
Zhao, Fangfang
Li, Yinpeng
Cui, Mingda
Wang, Haiyan
Meng, Xianghui
Cai, Shanbao
author_sort Pu, Youguang
collection PubMed
description BACKGROUND: Chemoresistance hinders the curative cancer chemotherapy. MicroRNAs (miRNAs) are key players in diverse biological processes including the chemoresistance of cancers. METHODS: A RNA-seq-based miR-omic analysis of osteosarcoma (OS) cells was performed to detect the levels of miR-34a-5p. Bioinformatics analysis revealed that AGTR1 is one of the target genes of miR-34a-5p. The mRNA and protein levels of AGTR1 were detected in both the miR-34a-5p-mimic transfected G-292 and miR-34a-5p-antagomiR transfected SJSA-1 cells. The involvement of AGTR1 with OS chemoresistance was validated by the experiments with siRNA-mediated repression or overexpression of the AGTR1 gene. RESULTS: We showed that miR-34a-5p promotes the multi- chemoresistance of OS. The angiotensin II type 1 receptor (AGTR1) gene, is one of the targets of miR-34a-5p in OS and thus negatively correlates with OS chemoresistance by systematic investigations of a multi-drug sensitive (G-292) and resistant (SJSA-1) OS cell lines. Down-regulation of the AGTR1 expression by siRNA passivates G-292 cells and suppresses cell apoptosis, while over-expression of AGTR1 sensitizes SJSA-1 cells and thus promotes the drug-triggered cell death. CONCLUSIONS: The miR-34a-5p and its target gene AGTR1 are the potential targets for an effective chemotherapy of OS. Our results also provide novel insights into the effective chemotherapy for OS patients. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12885-016-3002-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-52233222017-01-11 The miR-34a-5p promotes the multi-chemoresistance of osteosarcoma via repression of the AGTR1 gene Pu, Youguang Zhao, Fangfang Li, Yinpeng Cui, Mingda Wang, Haiyan Meng, Xianghui Cai, Shanbao BMC Cancer Research Article BACKGROUND: Chemoresistance hinders the curative cancer chemotherapy. MicroRNAs (miRNAs) are key players in diverse biological processes including the chemoresistance of cancers. METHODS: A RNA-seq-based miR-omic analysis of osteosarcoma (OS) cells was performed to detect the levels of miR-34a-5p. Bioinformatics analysis revealed that AGTR1 is one of the target genes of miR-34a-5p. The mRNA and protein levels of AGTR1 were detected in both the miR-34a-5p-mimic transfected G-292 and miR-34a-5p-antagomiR transfected SJSA-1 cells. The involvement of AGTR1 with OS chemoresistance was validated by the experiments with siRNA-mediated repression or overexpression of the AGTR1 gene. RESULTS: We showed that miR-34a-5p promotes the multi- chemoresistance of OS. The angiotensin II type 1 receptor (AGTR1) gene, is one of the targets of miR-34a-5p in OS and thus negatively correlates with OS chemoresistance by systematic investigations of a multi-drug sensitive (G-292) and resistant (SJSA-1) OS cell lines. Down-regulation of the AGTR1 expression by siRNA passivates G-292 cells and suppresses cell apoptosis, while over-expression of AGTR1 sensitizes SJSA-1 cells and thus promotes the drug-triggered cell death. CONCLUSIONS: The miR-34a-5p and its target gene AGTR1 are the potential targets for an effective chemotherapy of OS. Our results also provide novel insights into the effective chemotherapy for OS patients. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12885-016-3002-x) contains supplementary material, which is available to authorized users. BioMed Central 2017-01-10 /pmc/articles/PMC5223322/ /pubmed/28073349 http://dx.doi.org/10.1186/s12885-016-3002-x Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Pu, Youguang
Zhao, Fangfang
Li, Yinpeng
Cui, Mingda
Wang, Haiyan
Meng, Xianghui
Cai, Shanbao
The miR-34a-5p promotes the multi-chemoresistance of osteosarcoma via repression of the AGTR1 gene
title The miR-34a-5p promotes the multi-chemoresistance of osteosarcoma via repression of the AGTR1 gene
title_full The miR-34a-5p promotes the multi-chemoresistance of osteosarcoma via repression of the AGTR1 gene
title_fullStr The miR-34a-5p promotes the multi-chemoresistance of osteosarcoma via repression of the AGTR1 gene
title_full_unstemmed The miR-34a-5p promotes the multi-chemoresistance of osteosarcoma via repression of the AGTR1 gene
title_short The miR-34a-5p promotes the multi-chemoresistance of osteosarcoma via repression of the AGTR1 gene
title_sort mir-34a-5p promotes the multi-chemoresistance of osteosarcoma via repression of the agtr1 gene
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223322/
https://www.ncbi.nlm.nih.gov/pubmed/28073349
http://dx.doi.org/10.1186/s12885-016-3002-x
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