The synthesis of (15)N(7)-Hoogsteen face-labeled adenosine phosphoramidite for solid-phase RNA synthesis

ABSTRACT: We have developed an efficient route for the synthesis of (15)N(7)-labeled adenosine as phosphoramidite building block for site- and atom-specific incorporation into RNA by automated solid-phase synthesis. Such labeled RNA is required for the evaluation of selected non-canonical base pair...

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Detalles Bibliográficos
Autores principales: Neuner, Sandro, Kreutz, Christoph, Micura, Ronald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2016
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225212/
https://www.ncbi.nlm.nih.gov/pubmed/28127100
http://dx.doi.org/10.1007/s00706-016-1882-8
Descripción
Sumario:ABSTRACT: We have developed an efficient route for the synthesis of (15)N(7)-labeled adenosine as phosphoramidite building block for site- and atom-specific incorporation into RNA by automated solid-phase synthesis. Such labeled RNA is required for the evaluation of selected non-canonical base pair interactions in folded RNA using NMR spectroscopic methods. GRAPHICAL ABSTRACT: [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00706-016-1882-8) contains supplementary material, which is available to authorized users.

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