The synthesis of (15)N(7)-Hoogsteen face-labeled adenosine phosphoramidite for solid-phase RNA synthesis

ABSTRACT: We have developed an efficient route for the synthesis of (15)N(7)-labeled adenosine as phosphoramidite building block for site- and atom-specific incorporation into RNA by automated solid-phase synthesis. Such labeled RNA is required for the evaluation of selected non-canonical base pair...

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Detalles Bibliográficos
Autores principales: Neuner, Sandro, Kreutz, Christoph, Micura, Ronald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2016
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225212/
https://www.ncbi.nlm.nih.gov/pubmed/28127100
http://dx.doi.org/10.1007/s00706-016-1882-8
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author Neuner, Sandro
Kreutz, Christoph
Micura, Ronald
author_facet Neuner, Sandro
Kreutz, Christoph
Micura, Ronald
author_sort Neuner, Sandro
collection PubMed
description ABSTRACT: We have developed an efficient route for the synthesis of (15)N(7)-labeled adenosine as phosphoramidite building block for site- and atom-specific incorporation into RNA by automated solid-phase synthesis. Such labeled RNA is required for the evaluation of selected non-canonical base pair interactions in folded RNA using NMR spectroscopic methods. GRAPHICAL ABSTRACT: [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00706-016-1882-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-52252122017-01-24 The synthesis of (15)N(7)-Hoogsteen face-labeled adenosine phosphoramidite for solid-phase RNA synthesis Neuner, Sandro Kreutz, Christoph Micura, Ronald Monatsh Chem Original Paper ABSTRACT: We have developed an efficient route for the synthesis of (15)N(7)-labeled adenosine as phosphoramidite building block for site- and atom-specific incorporation into RNA by automated solid-phase synthesis. Such labeled RNA is required for the evaluation of selected non-canonical base pair interactions in folded RNA using NMR spectroscopic methods. GRAPHICAL ABSTRACT: [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00706-016-1882-8) contains supplementary material, which is available to authorized users. Springer Vienna 2016-12-08 2017 /pmc/articles/PMC5225212/ /pubmed/28127100 http://dx.doi.org/10.1007/s00706-016-1882-8 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Paper
Neuner, Sandro
Kreutz, Christoph
Micura, Ronald
The synthesis of (15)N(7)-Hoogsteen face-labeled adenosine phosphoramidite for solid-phase RNA synthesis
title The synthesis of (15)N(7)-Hoogsteen face-labeled adenosine phosphoramidite for solid-phase RNA synthesis
title_full The synthesis of (15)N(7)-Hoogsteen face-labeled adenosine phosphoramidite for solid-phase RNA synthesis
title_fullStr The synthesis of (15)N(7)-Hoogsteen face-labeled adenosine phosphoramidite for solid-phase RNA synthesis
title_full_unstemmed The synthesis of (15)N(7)-Hoogsteen face-labeled adenosine phosphoramidite for solid-phase RNA synthesis
title_short The synthesis of (15)N(7)-Hoogsteen face-labeled adenosine phosphoramidite for solid-phase RNA synthesis
title_sort synthesis of (15)n(7)-hoogsteen face-labeled adenosine phosphoramidite for solid-phase rna synthesis
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225212/
https://www.ncbi.nlm.nih.gov/pubmed/28127100
http://dx.doi.org/10.1007/s00706-016-1882-8
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