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Thylakoids reduce body fat and fat cell size by binding to dietary fat making it less available for absorption in high-fat fed mice
BACKGROUND: Dietary thylakoids derived from spinach have beneficial effects on body fat accumulation and blood lipids as demonstrated in humans and rodents. Important mechanisms established include delayed fat digestion in the intestine, without causing steatorrhea, and increased fatty acid oxidatio...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225541/ https://www.ncbi.nlm.nih.gov/pubmed/28096887 http://dx.doi.org/10.1186/s12986-016-0160-4 |
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author | Stenkula, Karin G. Stenblom, Eva-Lena Montelius, Caroline Egecioglu, Emil Erlanson-Albertsson, Charlotte |
author_facet | Stenkula, Karin G. Stenblom, Eva-Lena Montelius, Caroline Egecioglu, Emil Erlanson-Albertsson, Charlotte |
author_sort | Stenkula, Karin G. |
collection | PubMed |
description | BACKGROUND: Dietary thylakoids derived from spinach have beneficial effects on body fat accumulation and blood lipids as demonstrated in humans and rodents. Important mechanisms established include delayed fat digestion in the intestine, without causing steatorrhea, and increased fatty acid oxidation in intestinal cells. The objective of our study was to elucidate if increased fecal fat excretion is an important mechanism to normalize adipose tissue metabolism during high-fat feeding in mice supplemented with thylakoids. METHODS: Mice were randomized to receive HFD or thylHFD for 14 days (n = 14 for the control group and 16 for the thylakoid group). The effect of thylakoids on body fat distribution, faecal and liver fat content, and adipose tissue metabolism was investigated following high-fat feeding. RESULTS: Thylakoid supplementation for 14 days caused an increased faecal fat content without compensatory eating compared to control. As a result, thylakoid treated animals had reduced fat mass depots and reduced liver fat accumulation compared to control. The size distribution of adipocytes isolated from visceral adipose tissue was narrowed and the cell size decreased. Adipocytes isolated from thylakoid-treated mice displayed a significantly increased lipogenesis, and protein expression of peroxisome proliferator-activated receptor gamma (PPARγ), down-stream target FAS, as well as transcription factor coactivators PGC1-α and LPIN-1 were upregulated in adipose tissue from thylakoid-fed mice. CONCLUSIONS: Together, these data suggest that thylakoid supplementation reduces body fat and fat cell size by binding to dietary fat and increasing its fecal excretion, thus reducing dietary fat available for absorption. |
format | Online Article Text |
id | pubmed-5225541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-52255412017-01-17 Thylakoids reduce body fat and fat cell size by binding to dietary fat making it less available for absorption in high-fat fed mice Stenkula, Karin G. Stenblom, Eva-Lena Montelius, Caroline Egecioglu, Emil Erlanson-Albertsson, Charlotte Nutr Metab (Lond) Research BACKGROUND: Dietary thylakoids derived from spinach have beneficial effects on body fat accumulation and blood lipids as demonstrated in humans and rodents. Important mechanisms established include delayed fat digestion in the intestine, without causing steatorrhea, and increased fatty acid oxidation in intestinal cells. The objective of our study was to elucidate if increased fecal fat excretion is an important mechanism to normalize adipose tissue metabolism during high-fat feeding in mice supplemented with thylakoids. METHODS: Mice were randomized to receive HFD or thylHFD for 14 days (n = 14 for the control group and 16 for the thylakoid group). The effect of thylakoids on body fat distribution, faecal and liver fat content, and adipose tissue metabolism was investigated following high-fat feeding. RESULTS: Thylakoid supplementation for 14 days caused an increased faecal fat content without compensatory eating compared to control. As a result, thylakoid treated animals had reduced fat mass depots and reduced liver fat accumulation compared to control. The size distribution of adipocytes isolated from visceral adipose tissue was narrowed and the cell size decreased. Adipocytes isolated from thylakoid-treated mice displayed a significantly increased lipogenesis, and protein expression of peroxisome proliferator-activated receptor gamma (PPARγ), down-stream target FAS, as well as transcription factor coactivators PGC1-α and LPIN-1 were upregulated in adipose tissue from thylakoid-fed mice. CONCLUSIONS: Together, these data suggest that thylakoid supplementation reduces body fat and fat cell size by binding to dietary fat and increasing its fecal excretion, thus reducing dietary fat available for absorption. BioMed Central 2017-01-11 /pmc/articles/PMC5225541/ /pubmed/28096887 http://dx.doi.org/10.1186/s12986-016-0160-4 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Stenkula, Karin G. Stenblom, Eva-Lena Montelius, Caroline Egecioglu, Emil Erlanson-Albertsson, Charlotte Thylakoids reduce body fat and fat cell size by binding to dietary fat making it less available for absorption in high-fat fed mice |
title | Thylakoids reduce body fat and fat cell size by binding to dietary fat making it less available for absorption in high-fat fed mice |
title_full | Thylakoids reduce body fat and fat cell size by binding to dietary fat making it less available for absorption in high-fat fed mice |
title_fullStr | Thylakoids reduce body fat and fat cell size by binding to dietary fat making it less available for absorption in high-fat fed mice |
title_full_unstemmed | Thylakoids reduce body fat and fat cell size by binding to dietary fat making it less available for absorption in high-fat fed mice |
title_short | Thylakoids reduce body fat and fat cell size by binding to dietary fat making it less available for absorption in high-fat fed mice |
title_sort | thylakoids reduce body fat and fat cell size by binding to dietary fat making it less available for absorption in high-fat fed mice |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225541/ https://www.ncbi.nlm.nih.gov/pubmed/28096887 http://dx.doi.org/10.1186/s12986-016-0160-4 |
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