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High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii

Systematic identification and characterization of genetic perturbations have proven useful to decipher gene function and cellular pathways. However, the conventional approaches of permanent gene deletion cannot be applied to essential genes. We have pioneered a unique collection of ~70 temperature-s...

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Detalles Bibliográficos
Autores principales: Breker, Michal, Lieberman, Kristi, Tulin, Frej, Cross, Frederick R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226362/
https://www.ncbi.nlm.nih.gov/pubmed/28060315
http://dx.doi.org/10.3791/54831