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Native ESI Mass Spectrometry Can Help to Avoid Wrong Interpretations from Isothermal Titration Calorimetry in Difficult Situations

We studied by native ESI-MS the binding of various DNA-polymerase-derived peptides onto DNA-polymerase processivity rings from Escherichia coli, Pseudomonas aeruginosa, and Mycobacterium tuberculosis. These homodimeric rings present two equivalent specific binding sites, which leads to successive fo...

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Detalles Bibliográficos
Autores principales: Wolff, Philippe, Da Veiga, Cyrielle, Ennifar, Eric, Bec, Guillaume, Guichard, Gilles, Burnouf, Dominique, Dumas, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5227004/
https://www.ncbi.nlm.nih.gov/pubmed/27957716
http://dx.doi.org/10.1007/s13361-016-1534-6