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Networks of Cultured iPSC-Derived Neurons Reveal the Human Synaptic Activity-Regulated Adaptive Gene Program

Long-term adaptive responses in the brain, such as learning and memory, require synaptic activity-regulated gene expression, which has been thoroughly investigated in rodents. Using human iPSC-derived neuronal networks, we show that the human and the mouse synaptic activity-induced transcriptional p...

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Detalles Bibliográficos
Autores principales: Pruunsild, Priit, Bengtson, C. Peter, Bading, Hilmar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5236011/
https://www.ncbi.nlm.nih.gov/pubmed/28052243
http://dx.doi.org/10.1016/j.celrep.2016.12.018
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author Pruunsild, Priit
Bengtson, C. Peter
Bading, Hilmar
author_facet Pruunsild, Priit
Bengtson, C. Peter
Bading, Hilmar
author_sort Pruunsild, Priit
collection PubMed
description Long-term adaptive responses in the brain, such as learning and memory, require synaptic activity-regulated gene expression, which has been thoroughly investigated in rodents. Using human iPSC-derived neuronal networks, we show that the human and the mouse synaptic activity-induced transcriptional programs share many genes and both require Ca(2+)-regulated synapse-to-nucleus signaling. Species-specific differences include the noncoding RNA genes BRE-AS1 and LINC00473 and the protein-coding gene ZNF331, which are absent in the mouse genome, as well as several human genes whose orthologs are either not induced by activity or are induced with different kinetics in mice. These results indicate that lineage-specific gain of genes and DNA regulatory elements affects the synaptic activity-regulated gene program, providing a mechanism driving the evolution of human cognitive abilities.
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spelling pubmed-52360112017-01-24 Networks of Cultured iPSC-Derived Neurons Reveal the Human Synaptic Activity-Regulated Adaptive Gene Program Pruunsild, Priit Bengtson, C. Peter Bading, Hilmar Cell Rep Article Long-term adaptive responses in the brain, such as learning and memory, require synaptic activity-regulated gene expression, which has been thoroughly investigated in rodents. Using human iPSC-derived neuronal networks, we show that the human and the mouse synaptic activity-induced transcriptional programs share many genes and both require Ca(2+)-regulated synapse-to-nucleus signaling. Species-specific differences include the noncoding RNA genes BRE-AS1 and LINC00473 and the protein-coding gene ZNF331, which are absent in the mouse genome, as well as several human genes whose orthologs are either not induced by activity or are induced with different kinetics in mice. These results indicate that lineage-specific gain of genes and DNA regulatory elements affects the synaptic activity-regulated gene program, providing a mechanism driving the evolution of human cognitive abilities. Cell Press 2017-01-03 /pmc/articles/PMC5236011/ /pubmed/28052243 http://dx.doi.org/10.1016/j.celrep.2016.12.018 Text en © 2017 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Pruunsild, Priit
Bengtson, C. Peter
Bading, Hilmar
Networks of Cultured iPSC-Derived Neurons Reveal the Human Synaptic Activity-Regulated Adaptive Gene Program
title Networks of Cultured iPSC-Derived Neurons Reveal the Human Synaptic Activity-Regulated Adaptive Gene Program
title_full Networks of Cultured iPSC-Derived Neurons Reveal the Human Synaptic Activity-Regulated Adaptive Gene Program
title_fullStr Networks of Cultured iPSC-Derived Neurons Reveal the Human Synaptic Activity-Regulated Adaptive Gene Program
title_full_unstemmed Networks of Cultured iPSC-Derived Neurons Reveal the Human Synaptic Activity-Regulated Adaptive Gene Program
title_short Networks of Cultured iPSC-Derived Neurons Reveal the Human Synaptic Activity-Regulated Adaptive Gene Program
title_sort networks of cultured ipsc-derived neurons reveal the human synaptic activity-regulated adaptive gene program
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5236011/
https://www.ncbi.nlm.nih.gov/pubmed/28052243
http://dx.doi.org/10.1016/j.celrep.2016.12.018
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