Construction of Gpm6a/Reelin(GFPCreERT2) by BAC recombination using a specific gene in hepatic mesothelial or stellate cells

AIM: To prepare a Gpm6a/Reelin(GFPCreERT2) construct with a rapid and reliable strategy using a bacterial artificial chromosome (BAC). METHODS: Gpm6a and Reelin BACs were purified and transformed into SW102 E. coli by electroporation. The GFPCreERT2 fragment was prepared from a shuttle vector and tr...

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Detalles Bibliográficos
Autores principales: Shi, Hong-Bo, Lou, Jin-Li, Shi, Hong-Lin, Ren, Feng, Chen, Yu, Duan, Zhong-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2017
Materias:
Basic Study
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5236502/
https://www.ncbi.nlm.nih.gov/pubmed/28127196
http://dx.doi.org/10.3748/wjg.v23.i2.224

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5236502/
https://www.ncbi.nlm.nih.gov/pubmed/28127196
http://dx.doi.org/10.3748/wjg.v23.i2.224

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