Cargando…
Construction of Gpm6a/Reelin(GFPCreERT2) by BAC recombination using a specific gene in hepatic mesothelial or stellate cells
AIM: To prepare a Gpm6a/Reelin(GFPCreERT2) construct with a rapid and reliable strategy using a bacterial artificial chromosome (BAC). METHODS: Gpm6a and Reelin BACs were purified and transformed into SW102 E. coli by electroporation. The GFPCreERT2 fragment was prepared from a shuttle vector and tr...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Baishideng Publishing Group Inc
2017
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5236502/ https://www.ncbi.nlm.nih.gov/pubmed/28127196 http://dx.doi.org/10.3748/wjg.v23.i2.224 |